Quantitative coherent anti-Stokes Raman scattering microspectroscopy using a nanosecond supercontinuum light source |
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| Authors: | Masanari Okuno Hideaki Kano Philippe Leproux Vincent Couderc Hiro-o Hamaguchi |
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| Affiliation: | 1. Department of Chemistry, School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0022, Japan;2. Institut de Recherche XLIM, UMR CNRS 6172, 123 Avenue Albert Thomas, 87060 Limoges Cedex, France;3. LEUKOS, ESTER Technopole, 1 Avenue d’Ester, 87069 Limoges Cedex, France;4. Institute of Molecular Science and Department of Applied Chemistry, National Chiao Tung University, 1001 Ta Hsueh Road, Hsinchu 300, Taiwan;1. philippe.leproux@unilim.fr;2. john.dudley@univ-fcomte.fr;3. mni@umich.edu;4. jr.taylor@ic.ac.uk;5. hkano@chem.s.u-tokyo.ac.jp |
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| Abstract: | We describe and characterize a multiplex CARS microspectroscopic system that uses a nanosecond supercontinuum generated from a photonic crystal fiber and a sub-nanosecond pulse laser. This system has a high spectral resolution (<0.1 cm?1) and an ultrabroadband spectral coverage (>2500 cm?1). The estimated spatial resolutions are 0.45 μm (lateral) and 4.5 μm (axial), respectively. This system enables us to obtain CARS spectra and corresponding images in the fingerprint region as well in the CH stretch region. Using this system, we have successfully obtained label-free and multi-mode vibrational images of a yeast cell. |
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