纳米羟基磷灰石丝素蛋白仿生矿化材料的制备研究

以Ca(NO₃)₂与Na₃PO₄为无机相的前驱体，将丝素蛋白直接溶于Ca(NO₃)₂溶液中，不经过脱盐处理，直接滴入Na₃PO₄溶液中反应，在37℃下丝素蛋白和羟基磷灰石晶体之间相互作用，仿生合成了纳米羟基磷灰石(n-HA)丝素蛋白(SF)生物矿化材料.用FTIR、XRD、XPS和SEM进行表征.结果表明，羟基磷灰石和丝素蛋白两相间具有较强的化学键合，矿化材料中无机相包含少量碳酸根，为缺钙类骨羟基磷灰石并且呈现一定的长轴取向性，说明丝素蛋白大分子对羟基磷灰石晶体的成核和生长起着模板和调控作用.矿化物颗粒尺寸在50~200nm之间，其抗压强度为32.21MPa，可作为非承重部位骨组织缺损修复材料.

Study on Preparation of n-HA and Silk Fibroin Bio-mineral Material

A new hydroxyapatite (n-HA) Silk fibroin (SF) bio-mineral material was prepared by using a biomimetic method, with Ca(NO₃)₂ and Na₃PO₄ used as the starting reagent to synthesize the inorganic phase. In details, SF was dissolved in Ca(NO₃)₂ solution without desalting procedure, the compound solution was directly dropped into Na₃PO₄ solution. In this way, HA was synthesized at 37℃ by controlling Ca/P ratio, meanwhile the minerialized HA interacted with SF to form biomineral material. The morphology and structure of this n-HA-SF biomineral material were characterized by XRD, FTIR, XPS and SEM. The results show that there are strong chemical interaction between n-HA and SF. Moreover, in the biomineral material, the inorganic phase is calcium deficient hydroxyapatite, containing a small amount of carbonated HA with an average crystal size of 37.6nm. In addition, The particle size of biomineral materials range from 50nm to 200nm.It can be deduced that silk fibroin chain can regulate the growth and morphology of HA crystals. The compressive strength of the biomineral material is 32.21MPa， which is a good potential material for bone tissue engineering and unloaded bearing bone defects repairing.