| 电离辐射对EL-4细胞的P53蛋白及其下游基因MDM2 mRNA和蛋白表达的影响 |
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| 引用本文： | 傅海青,罗灿,傅士波,鞠桂芝.电离辐射对EL-4细胞的P53蛋白及其下游基因MDM2 mRNA和蛋白表达的影响[J].辐射研究与辐射工艺学报,2000,18(3):208-211. |
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| 作者姓名： | 傅海青 罗灿 傅士波 鞠桂芝 |
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| 作者单位： | 白求恩医科大学卫生部放射生物学重点实验室长春 130021 |
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| 基金项目： | 国家自然科学基金资助!（39770193） |
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| 摘 要： | 采用流式细胞术和Ｎｏｒｔｈｅｒｎ ｂｌｏｔ杂交检测了Ｘ射线照射后ＥＬ－４细胞中ｐ５３蛋白及其下游基因ＭＤＭ２ｍＲＮＡ和蛋白表达的变化，以探讨Ｘ射线照射诱导ＥＬ－４细胞Ｇ１期阻滞的分子机制。结果表明：经４ＧｙＸ射线照射后的ＥＬ－４细胞，ｐ５３蛋白表达在照射后２ｈ增高，４ｈ达峰值，持续至照射后２４ｈ（ｐ〈０．０５～０．００１）；ＭＤＭ２蛋白的表达在照射后４～２４ｈ明显升高（ｐ〈０．０５～０．０１）。进
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| 关 键 词： | 电离辐射 EL－4细胞 MDM2 p53蛋白 基因表达 |
| 修稿时间： | 1999年11月16日 |
Effect of ionizing radiation on p53 and MDM2 gene expressions of EL-4 cells |
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| FU Haiqing,LUO Can FU Shibo,JU Guizhi.Effect of ionizing radiation on p53 and MDM2 gene expressions of EL-4 cells[J].Journal of Radiation Research and Radiation Processing,2000,18(3):208-211. |
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| Authors: | FU Haiqing LUO Can FU Shibo JU Guizhi |
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| Abstract: | To explore the molecular mechanism of G1 arrest of EL-4 cells induced by whole body irradiation (WBI), Flow cytometry (FCM) and Northern blot were used to analyze p53, MDM2 gene expressions of EL-4 cells after irradiation with X-rays. Results showed that the p53 protein level of E1-4 cells increased from 2 to 24h, and the MDM2 protein level of E1-4 increased from 4 to 24h after 4Gy irradiation with X-rays. Meanwhile, the MDM2 mRNA level was sjgnificantly increased at 4h after irradiation. Conclusion: these results suggested that there was a feedback loop between p53 and MDM2 protein in EL-4 cells after irradiation with X-rays, which made G1 arrest cells reenter cell cycle. |
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| Keywords: | Ionizing radiation EL-4 cells MDM2 p53 |
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