Long-term stable hydrogen production from acetate using immobilized Rhodobacter capsulatus in a panel photobioreactor |
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| Affiliation: | 1. Department of Biology and Biotechnology, Islamic University of Gaza, Gaza, Palestine;2. Department of Biological Sciences, Middle East Technical University, Ankara 06800, Turkey;3. Department of Biology, Faculty of Arts and Sciences, Osmaniye Korkut Ata University, Osmaniye 80000, Turkey;4. Department of Chemical Engineering, Faculty of Engineering, University of Maragheh, P. O. Box 55181-83111, Maragheh, Iran;5. Department of Chemical Engineering, Middle East Technical University, Ankara 06800, Turkey;1. Research Institute of Biology, Biology Faculty, Yerevan State University, 1 A. Manoogian Str., 0025 Yerevan, Armenia;2. Department of Biochemistry, Microbiology and Biotechnology, Biology Faculty, Yerevan State University, 1 A. Manoogian Str., 0025 Yerevan, Armenia;1. Department of Biotechnology, Middle East Technical University, 06800 Ankara, Turkey;2. Department of Chemical Engineering, Middle East Technical University, 06800 Ankara, Turkey;3. Department of Biological Sciences, Middle East Technical University, 06800 Ankara, Turkey;1. State Key Laboratory of Multiphase Flow in Power Engineering, Xi''an Jiaotong University, Xian 710049, Shaanxi, PR China;2. Department of Environmental Science & Engineering, Xi''an Jiaotong University, Xian 710049, Shaanxi, PR China;1. Department of Biological Sciences, Middle East Technical University, Ankara, 06531, Turkey;2. Department of Molecular Biology and Genetics, Faculty of Science, Necmettin Erbakan University, Konya, Turkey;3. Department of Chemical Engineering, Middle East Technical University, Ankara, 06531, Turkey;1. Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, 400044, China;2. Institute of Engineering Thermophysics, Chongqing University, Chongqing 400044, China;3. Chongqing Key Laboratory of Modern Photoelectric Detection Technology and Instrument, Chongqing University of Technology, 400044, China |
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| Abstract: | Biological hydrogen production is attractive since renewable resources are utilized for hydrogen production. In this study, a novel panel photobioreactor (1.4 L) was constructed from Plexiglas with a network of nylon fabric support for agar immobilized bacteria complex. Two strains of Rhodobacter capsulatus DSM 1710 wild-type strain and Rhodobacter capsulatus YO3 (hup−, uptake hydrogenase deleted mutant) with cell concentrations of 2.5 and 5.0 mg dcw/mL agar, respectively were entrapped by 4% (w/v) of agar. The system was operated for 72–82 days in a sequential batch mode utilizing acetate as substrate at 30 °C under continuous illumination. Immobilization increased the stability of the photobioreactors by reducing the fluctuations in pH. The pH remained between 6.7 and 8.0 during the process. Both hydrogen yield and productivity were higher in immobilized photobioreactors compared to suspended culture. The highest hydrogen productivities of 0.75 mmol H2/L/h and 1.3 mmol H2/L/h were obtained by R. capsulatus DSM1710 and R. capsulatus YO3 respectively. |
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| Keywords: | Biological hydrogen production Agar immobilized bacteria Photobioreactor Sequential batch process |
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