The effect of hydrolysis and sterilization in biohydrogen production from cassava processing wastewater medium using anaerobic bacterial consortia |
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| Affiliation: | 1. Federal University of Paraná, Department of Bioprocess Engineering and Biotechnology, Centro Politécnico, 81531-990, Curitiba, Paraná, Brazil;2. Federal University of Technology – Paraná, Department of Bioprocess Engineering and Biotechnology, 84016-210, Ponta Grossa, Paraná, Brazil;3. Department of Civil Engineering, Lassonde School of Engineering, York University, Bergeron Centre for Engineering Excellence, 11 Aboretum Lane, York University, Toronto, ON, M3J 1P3, Canada;1. Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaí́so, Av Brasil 2085, 234-0950, Valparaı́so, Chile;2. Faculty of Engineering and Science, Universidad Adolfo Ibáñez, Avda Padre Hurtado 750, Viña del Mar, Chile;1. Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, 230031, China;2. Science Island Branch of Graduate, University of Science & Technology of China, Hefei, 230031, China;1. Bioprocess Engineering and Biotechnology Department, Federal University of Paraná, Centro Politécnico, CP 19011, Curitiba-PR 81531-908, Brazil;2. Chemistry Department, University of Guadalajara, Mexico Centro Universitario de Ciencias Exactas e Ingenierías, CP 44430 Guadalajara-Jalisco, Mexico;3. Chemical Engineering Department, Federal University of Paraná, Centro Politécnico, CP 19011, Curitiba-PR 81531-908, Brazil |
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| Abstract: | We evaluated the production of bioH2 from Cassava Processing Wastewater (CPW) using three microbial consortia (Vac, Esg, and Lod) from different Brazilian environments. These consortia consisted of bacteria of the genera Clostridium, Sporanaerobacter, Coprococcus, Enterococcus, and others. The CPW was supplemented with nitrogen and used raw or hydrolyzed and sterilized or not. Four independent variables were optimized (Box-Behnken design): pH, temperature, C/N ratio, and inoculum ratio. Three quadratic models were obtained and explain production of bioH2 (R2 of 0.93, 0.87 and 0.82 for the consortia Vac, Esg and Lod, respectively). The quadratic effects were the most significant in comparison to linear effects and interactions. The optimal conditions were: pH: 5.5–7.0; temperature 37-39 °C; inoculum ratio 15%, and C/N ratio 5-3,5. After 48 h, the maximum yields of hydrogen obtained with hydrolyzed and sterilized CPW were 1.82, 1.7 and 1.68 mols of H2/mol of maltose for Lod, Esg and Vac, respectively. While, for the only sterilized substrate the yields are in the range 1.33–1.54 mol H2/mol maltose. |
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| Keywords: | Cassava processing wastewater Consortia Optimization Dark fermentation |
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