Effect of cornstalk hydrolysis on photo-fermentative hydrogen production by R. capsulatus |
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| Affiliation: | 1. School of Chemistry and Chemical Engineering, South China University of Technology; Guangdong Key Lab for Fuel Cell Technology, Guangzhou 510641, China;2. School of Materials Science and Engineering, Georgia Institute of Technology, 771 Ferst Drive, Atlanta, GA 30332-0245, United States;1. State Key Laboratory of Petroleum Resources and Prospecting, China University of Petroleum at Beijing, Changping, 100249, China;2. The University of Texas at Austin, Austin, TX, 78712, USA;1. Chemistry Department, Faculty of Science, Fayoum University, Fayoum, Egypt;2. Chemistry Department, College of Science & Arts in Qurayat, Jouf University, Saudi Arabia;1. Department of Chemical Engineering, Indian Institute of Technology Hyderabad, Kandi, Sangareddy, 502285, Telangana, India;2. Department of Chemistry, Indian Institute of Technology Hyderabad, Kandi, Sangareddy, 502285, Telangana, India |
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| Abstract: | Cornstalk is a typical cellulose material, which can be used by photo-fermentative H2 production after pretreatment. However, the pretreatment methods have different influence on photo fermentation. In this study, 25.0 g cornstalk was pretreated by HCl/NaOH/cellusase. The hydrolysis rates increased from 45.51% by ddH2O-treatment to 60.79% by diluted HCl-treatment and 51.6% by NaOH-treatment. The corresponding reducing sugar yields were 0.13 g/g, 0.42 g/g and 0.01 g/g, respectively. Enzymatic treatment enhanced the corresponding cornstalk hydrolysis rates to 50.81%, 67.60% and 64.10% with reducing sugar yields of 0.22 g/g, 0.62 g/g and 0.26 g/g. The sorts and concentrations of carbon source for H2 production vary among different hydrolysates. Photo-fermentative H2 production of strain R. capsulatus JL1 and mutant JL1601 (cheR2-) with hydrolysates were investigated. The maximum H2 yield of 123.8 ± 14.2 mL/g by strain JL1 was obtained from alkali-enzyme pretreated cornstalk, while the H2 yield of 224.9 ± 5.2 mL/g by mutant JL1601 (cheR2-) was obtained with acid-enzyme hydrolysate as the substrates. Meanwhile, the alkali pretreated cornstalk was the worst for photo-fermentation of both strain JL1 and mutant JL1601 (cheR2-). Nevertheless, the highest substrate conversion efficiencies for both strains were obtained from ddH2O-pretreated hydrolysate. Two-step pretreated hydrolysates were more beneficial to H2 production for mutant JL1601 (cheR2-) but not for strain JL1. |
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| Keywords: | Cornstalk hydrolysis Biohydrogen Photo-fermentation |
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