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Effects of size and autoclavation of fruit and vegetable wastes on biohydrogen production by dark dry anaerobic fermentation under mesophilic condition
Affiliation:1. Bioengineering Department, Faculty of Engineering, Ege University, 35100, Izmir, Turkey;2. Chemical Engineering Laboratory, Faculty of Sciences and Centre for Advanced Scientific Research (CICA), University of La Coruña, Rúa da Fraga 10, E–15008, A Coruña, Spain;3. Biology Department, Faculty of Science, Ege University, 35100, Izmir, Turkey;4. Application and Research Center for Testing and Analysis, Ege University, 35100, Izmir, Turkey;1. Department of Environmental Engineering, Daegu University, Gyeongsan, Gyeongbuk 712-714, South Korea;2. Center for Materials Cycles and Waste Management Research, National Institute for Environmental Studies, Tsukuba, Japan;3. Civil, Environmental and Architectural Engineering, Korea University, Anam-Dong, Seongbuk-gu, Seoul 136-714, South Korea;4. IT Convergence Materials R&D Group, Korea Institute of Industrial Technology, Chungnam 330-825, South Korea;5. Sustainable Environmental Process Research Institute, Daegu University, Gyeongsan, Gyeongbuk 712-714, South Korea;1. Department of Environmental Technology, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Av. Normalistas 800, Col. Colinas de la Normal, C.P. 44270, Guadalajara, Jalisco, Mexico;2. Department of Environmental Engineering and Water Technology, UNESCO-IHE Institute for Water Education, P. O. Box 3015, 2601 DA Delft, the Netherlands;1. Innovation Base of Groundwater and Environmental System Engineering, Chinese Research Academy of Environmental Science, Beijing 100012, China;2. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;1. Tsinghua University-Zhang Jiagang Joint Institute for Hydrogen Energy and Lithium-Ion Battery Technology, INET, Tsinghua University, Beijing 100084, PR China;2. Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing 100084, PR China;3. Beijing Key Laboratory of Radioactive Wastes Treatment, Tsinghua University, Beijing 100084, PR China;1. School of Architecture and Civil Engineering, Chengdu University, Chengdu 610106, China;2. Institute of New Energy and Low-carbon Technology, Sichuan University, Chengdu 610225, Sichuan, China;3. Key Lab of Northwest Water Resource, Environment and Ecology, MOE, Xi''an University of Architecture and Technology, Xi''an 710055, China;4. International Science & Technology Cooperation Center for Urban Alternative Water Resources Development, Xi''an 710055, China;5. Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia;6. Department of Civil and Environmental Engineering, Tohoku University, Sendai 9808579, Japan
Abstract:Fermentative hydrogen production from fruit and vegetable wastes (FVWs) through Dry Fermentation Technology (DFT) was studied through three independent experiments in order to find out the effect of particle size and autoclaving pretreatment on bio-hydrogen production from FVWs and as follows: (1) autoclaved FVWs with sizes < 5 cm (experiment I); (2) raw FVWs with sizes < 5 cm (experiment II) and (3) autoclaved FVWs with sizes > 5 cm (experiment III). The assay with autoclaved waste yielded a higher percentage of hydrogen in the headspace of the dry fermenter reaching a maximum value of 44% in experiment I. However, the maximum hydrogen production was obtained in experiment III with 14573 NmL at a yield of 23.53 NmL H2/gVS. Profiling of the microbial communities by denaturing gradient gel electrophoresis (DGGE) indicated that the most prominent species were the genera Clostridium, Bifidobacterium, and Lactobacillus.
Keywords:Dark dry anaerobic fermentation  Autoclaving  Biohydrogen  Fruit and vegetable wastes  Denaturing gradient gel electrophoresis (DGGE)
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