Improvement of biohydrogen production from glycerol in micro-oxidative environment |
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| Affiliation: | 1. LBE, Univ Montpellier, INRA, 102 avenue des Etangs, 11100, Narbonne, France;2. Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, Chile;1. Laboratory for Alternative Energy Conversion (LAEC), School of Mechatronic Systems Engineering, Simon Fraser University, Surrey, BC V3T 0A3, Canada;2. Automotive Fuel Cell Cooperation, 9000 Glenlyon Parkway, Burnaby, BC V5J 5J8, Canada;3. Department of Mechanical Engineering and Institute for Integrated Energy Systems, University of Victoria, Victoria V8P 5C2, BC, Canada;1. Centro de Investigación y Desarrollo Tecnológico en Electroquímica, Parque Tecnológico Querétaro S/N, Pedro Escobedo, Querétaro, 76703, Mexico;2. Énergie, Matériaux et Télécommunications (EMT), Institut National de La Recherche Scientifique (INRS), Université de Recherche, 1650 Boulevard Lionel Boulet, Varennes, Quebec, J3X 1S2, Canada;1. Research Centre for Sustainable Process Technology (CESPRO), Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor, Malaysia;2. Chemical Engineering Programme, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia;3. Sime Darby Research Sdn Bhd, R&D Centre – Carey Island, Lot 2664, Jalan Pulau Carey, 42960, Carey Island, Selangor, Malaysia;4. Department of Chemical Engineering, Feng Chia University, Taichung, 40724, Taiwan;1. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90112, Thailand;2. Department of Biology, Faculty of Science, Thaksin University, Phatthalung 93110, Thailand;3. Palm Oil Products and Technology Research Center (POPTEC), Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90112, Thailand |
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| Abstract: | Glycerol is a highly available by-product generated in the biodiesel industry. It can be converted into higher value products such as hydrogen using biological processes. The aim of this study was to optimize a continuous dark fermenter producing hydrogen from glycerol, by using micro-aerobic conditions to promote facultative anaerobes. For that, hydrogen peroxide (H2O2) was continuously added at low but constant flow rate (0.252 mL/min) with three different inlet concentrations (0.2, 0.4, and 0.6% w/w). A mixture of aerobic and anaerobic sludge was used as inoculum. Results showed that micro-oxidative environment significantly enhanced the overall hydrogen production. The maximum H2 yield (403.6 ± 94.7 mmolH2/molGlyconsumed) was reached at a H2O2 concentration of 0.6% (w/w), through the formate, ethanol and butyrate metabolic pathways. The addition of H2O2 promoted the development of facultative anaerobic microorganisms such as Klebsiella, Escherichia-Shigella and Enterococcus sp., likely by consuming oxygen traces in the medium and also producing hydrogen. Despite the micro-oxidative environment, strict anaerobes (Clostridium sp.) were still dominant in the microbial community and were probably the main hydrogen producing species. In conclusion, such micro-oxidative environment can improve hydrogen production by selecting specific microbial community structures with efficient metabolic pathways. |
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| Keywords: | Biohydrogen Dark fermentation Continuous stirred-tank reactor Mixed culture Glycerol |
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