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Biohydrogen production from autoclaved fruit and vegetable wastes by dry fermentation under thermophilic condition
Affiliation:1. Bioengineering Department, Faculty of Engineering, Ege University, 35100, Izmir, Turkey;2. Chemical Engineering Laboratory, Faculty of Sciences and Centre for Advanced Scientific Research (CICA), University of La Coruña, Rúa da Fraga 10, E – 15008 A Coruña, Spain;1. Institute of Advanced Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;2. Department of Biological and Agricultural Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;3. Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;4. Department of Agricultural Mechanization, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran;5. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan;1. Innovation Base of Groundwater and Environmental System Engineering, Chinese Research Academy of Environmental Science, Beijing 100012, China;2. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;1. College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China;2. School of Forestry, Northeast Forestry University, Harbin 150060, China;3. State Key Lab of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China;1. Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor, Malaysia;2. Department of Biotechnology-Biochemical Engineering, Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), P.O. Box 10, 50728, Gombak, Kuala Lumpur, Malaysia;3. Department of Chemical Engineering, Feng Chia University, Taichung 40724, Taiwan;1. College of Engineering, Nanjing Agricultural University, Nanjing, 210031, China;2. Department of Structures and Environmental Engineering, University of Agriculture, Faisalabad, Faisalabad, 38000, Pakistan;3. National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan;4. Department of Agricultural Engineering, PMAS Arid Agriculture University, Rawalpindi, Pakistan;5. Veterinary Research Institute, Lahore, Cantt, 54810, Pakistan;6. Institute of Food Science and Technology, University of Agriculture, Faisalabad, 38000, Faisalabad, Pakistan
Abstract:Dark fermentative hydrogen production from organic waste is an attractive technique that simultaneously treats waste along with generation of renewable fuel. In this study, a relative new technology named dark dry fermentation was tested in a 55-L reactor to treat fruit and vegetable waste (FVW) along with simultaneous generation of biohydrogen. To understand the effect of autoclaving as a pretreatment method on FVW for subsequent biohydrogen production, two independent experiments were performed; one with autoclaved waste (experiment I) and another by using non autoclaved waste (experiment II). From the analyses, it was found that maximum hydrogen % obtained for experiment I was 41% (v/v%) whereas, for experiment II was 21%. In terms of total hydrogen produced, around 30% higher production was observed with experiment I compared to experiment II. The hydrogen yields for experiment I and experiment II were respectively, 27.19 and 20.81 NmL H2/gVS (VS = volatile solid added), and the metabolites (VFAs) preferentially produced were acetic acid and iso-butyric acid.
Keywords:Biohydrogen  Autoclaving  Fruit and vegetable waste  Bioenergy
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