Effects of rice husk particle size on biohydrogen production under solid state fermentation |
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| Affiliation: | 1. Dokuz Eylül University, Biotechnology Department, Turkey;2. Dokuz Eylül University, Environmental Engineering Department, Turkey;1. University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan;2. Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan;3. Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan;1. Bioengineering Department, Faculty of Engineering, Ege University, 35100, Izmir, Turkey;2. Chemical Engineering Laboratory, Faculty of Sciences and Centre for Advanced Scientific Research (CICA), University of La Coruña, Rúa da Fraga 10, E – 15008 A Coruña, Spain;1. Dept. of Chemical Engineering, Feng Chia University, Taichung 40724, Taiwan;2. Green Energy Development Center, Feng Chia University, Taichung 40724, Taiwan;3. Master''s Program of Green Energy Science and Technology, Feng Chia University, Taiwan |
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| Abstract: | As a renewable energy source bio-hydrogen production from lignocellulosic wastes is a promising approach which can produce clean fuel with no CO2 emissions. Utilization of agro-industrial residues in solid state fermentation (SSF) is offering a solution to solid wastes disposal and providing an economical process of value-added products such as hydrogen.In this study three different particle size of rice husk (<2000 μm, <300 μm, <74 μm) was subjected to batch SSF with a Clostridium termitidis: Clostridium intestinale ratio of 5:1. C. termitidis is a cellulolytic microorganism that has the ability to hydrolyze cellulosic substances and C. intestinale is able to grow on glucose having a potential of enhancing hydrogen production when used in the co-culture. 5 g dw rice husk with 75% humidity was used as substrate in SSF under mesophilic conditions. The highest HF Volume (29.26 mL) and the highest yield (5.9 mL H2 g−1 substrate) were obtained with the smallest particle size (<74 μm). The main metabolites obtained from the fermentation media were acetic, butyric, propionic and lactic acids. The second best production yield (3.99 mL H2 g−1 substrate) was obtained with the middle particle size (<300 μm) rice husk with a HF of 19.71 mL. |
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| Keywords: | Biohydrogen Solid state fermentation Rice husk Combined fermentation |
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