Bioaugmentation with Thermoanaerobacterium thermosaccharolyticum W16 to enhance thermophilic hydrogen production using corn stover hydrolysate |
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| Affiliation: | 1. College of Power and Energy Engineering, Harbin Engineering University, Harbin 150001, China;2. State Key Laboratory of Urban Water Resources and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China;1. State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China;2. Department of Civil and Environmental Engineering, Tohoku University, Sendai 9808579, Japan;3. MaREI Centre, Environmental Research Institute, University College Cork, Cork, Ireland;4. School of Engineering, University College Cork, Cork, Ireland;1. Laboratory of Environmental Technology, INET, Tsinghua University, Beijing 100084, PR China;2. Beijing Key Laboratory of Radioactive Wastes Treatment, Tsinghua University, Beijing 100084, PR China;1. Centre for Bioresources and Biotechnology, TERI University, Plot No. 10, Institutional Area, New Delhi 110070, India;2. Microbial Biotechnology, Environmental and Industrial Biotechnology Division, The Energy and Resources Institute (TERI), India Habitat Centre, Darbari Seth Block, Lodhi Road, New Delhi 110003, India;1. Tsinghua University-Zhang Jiagang Joint Institute for Hydrogen Energy and Lithium-Ion Battery Technology, Tsinghua University, Beijing 100084, PR China;2. Beijing Key Laboratory of Radioactive Wastes Treatment, Tsinghua University, Beijing 100084, PR China |
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| Abstract: | In the present work, with corn stover hydrolysate as the substrate, an efficient hydrogen-producing thermophile, Thermoanaerobacterium thermosaccharolyticum W16, was added to three kinds of seed sludge (rotten corn stover (RCS), cow dung compost (CDC), and sludge from anaerobic digestion (SAD)) to investigate the effect of bioaugmentation on thermophilic hydrogen production. Batch test results indicate that the bioaugmentation with a small amount of the strain T. thermosaccharolyticum W16 (5% of total microbes) increased the hydrogen yield to varying degrees (RCS: from 8.78 to 9.90 mmol H2/g utilized sugar; CDC: from 8.18 to 8.42 mmol H2/g utilized sugar; SAD: from 8.55 to 9.17 mmol H2/g utilized sugar). The bioaugmentation process also influenced the soluble metabolites composition towards more acetate and less butyrate production for RCS, and more acetate and less ethanol accumulation for SAD. Microbial community analysis indicates that Thermoanaerobacterium spp. and Clostridium spp. dominated microbial community in all situations and might be mainly responsible for thermophilic hydrogen generation. For RCS and SAD, the bioaugmentation obviously increased the relative abundance of the strain T. thermosaccharolyticum W16 in microbial community, which might be the main reason for the improvement of hydrogen production in these cases. |
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| Keywords: | Biohydrogen Bioaugmentation Corn stover hydrolysate Seed sludge Microbial community |
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