High NH3N tolerance of a cheR2-deletion Rhodobacter capsulatus mutant for photo-fermentative hydrogen production using cornstalk |
| |
| Affiliation: | 1. State Key Laboratory of Multiphase Flow in Power Engineering, Xi''an Jiaotong University, Xian 710049, Shaanxi, PR China;2. Department of Environmental Science & Engineering, Xi''an Jiaotong University, Xian 710049, Shaanxi, PR China;1. Department of Biotechnology, Dr. B.R. Ambedkar National Institute of Technology, Jalandhar 144011, Punjab, India;2. Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, West Bengal, India;1. Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, CP6128 Succursale Centre-ville, Montréal, H3C 3J7 Québec, Canada;2. Faculty of Engineering, Department of Chemical Engineering, Ankara University, Tandogan, 06100 Ankara, Turkey;3. Life Sciences Research Center, Department of Biology, United States Air Force Academy, 2355 Faculty Drive, USAF Academy, 80840 CO, United States;1. División de Ciencias Ambientales, Instituto Potosino de Investigación Científica y Tecnológica, A.C. Camino a la Presa San José 2055, Lomas 4a Sección, C.P. 78216 San Luis Potosí, S.L.P., Mexico;2. LBE, INRA, 102 avenue des Etangs, 11100 Narbonne, France |
| |
| Abstract: | A mutant, JL1601, with a cheR2 gene deletion was constructed with Rhodobacter capsulatus JL1 as the wild type strain. The effects of pH, ammonia nitrogen concentration and light intensity on the photo-fermentative hydrogen production performance of JL1601 were studied. Suitable pH range for hydrogen production with the cheR2- mutant migrated from faintly acid to weak alkaline condition, and R.capsulatus JL1601 (cheR2-) showed higher NH3 N tolerance. The mutant performed better H2 production performance than the wildtype under the same illumination condition. The maximum H2 yield and rate of R.capsulatus JL1601 (cheR2-) obtained to be 4406.7 ± 45.9 mL/L and 54.1 ± 1.6 mL/(L·h) with the mixture of acetate and butyrate as the carbon sources, which were increased by 36.3% and 12.3% in comparison to those of wildtype, respectively. Practical experiments using acid-enzymatic pretreated cornstalk as the carbon source indicated that, the maximum hydrogen yields by the mutant JL1601 (cheR2-) was up to 224.85 ± 5.18 mL-H2/g-cornstalk, which increased by 171.43% compared with that of wild type strain. |
| |
| Keywords: | One-step photo-fermentation Cornstalk pretreatment Chemotaxis Gene knockout pH |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
