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Biohydrogen production from palm oil mill effluent (POME) by two stage anaerobic sequencing batch reactor (ASBR) system for better utilization of carbon sources in POME
Affiliation:1. Research Centre for Sustainable Process Technology (CESPRO), Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia (UKM), 43600 UKM, Bangi, Selangor, Malaysia;2. Chemical Engineering Programme, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor, Malaysia;1. Faculty of Engineering Technology, Universiti Malaysia Pahang (UMP), Lebuhraya Tun, Razak, 26300 Gambang, Kuantan, Pahang, Malaysia;2. College of Engineering and Technology, Northern Illinois University, 1425 W. Lincoln Highway, DeKalb, IL 60115-2828, United States;1. Department of Civil Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia;2. Department of Social and Preventive Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia;3. Institute of Ocean and Earth Sciences (IOES), University of Malaya, 50603 Kuala Lumpur, Malaysia;1. Department of Biology, Faculty of Science, Thaksin University, Patthalung 93110, Thailand;2. Microbial Resource Management Research Unit, Faculty of Science, Thaksin University, Patthalung 93110, Thailand;3. Biotechnology Program, Department of Biology, Faculty of Science, Thaksin University, Patthalung 93110, Thailand;4. Department of Biology and Centre for Geobiology, University of Bergen, P.O. Box 7800, N-5020, Bergen, Norway;1. Department of Civil Engineering, University of Malaya, 50603, Kuala Lumpur, Malaysia;2. Department of Applied Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran;3. Department of Mechanical Engineering, University of Malaya, 50603, Kuala Lumpur, Malaysia;1. Faculty of Engineering Technology, Universiti Malaysia Pahang (UMP), Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia;2. Faculty of Chemical Engineering Universiti Malaysia Pahang (UMP), Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia;3. Department of Engineering, University of Wisconsin – Stevens Point, 2001 Fourth Avenue, Stevens Point, WI 54481, USA
Abstract:The production of biohydrogen through dark fermentation of palm oil mill effluent (POME) was evaluated in two-stages of biohydrogen in an anaerobic sequencing batch reactor (ASBR) system using enriched mixed culture for the first time. This study attempts to examine the effect of HRT and its interaction behavior with the solid retention time (SRT), and the sugar consumption. The effluent after discharged from the thermophilic reactor contained 7.61 g/L TC and 22.87 g/L TSS was fed to the secondary mesophilic reactor system. Results indicated that the overall sugar consumption reached 88.62% at the optimum HRT of 12 h with the SRT set to 20 h. The optimum hydrogen yield and HPR in the thermophilic stage were 2.99 mol H2/mol-sugar and 8.54 mmol H2/L·h respectively, while for the mesophilic stage were 1.19 mol H2/mol-sugar and 1.47 mmolH2/L·h respectively. The overall HPR showed an improvement and increase from 8.54 mmol H2/L·h to 10.34 mmol H2/L.h. Microbial community analysis of mixed culture in the two-stage thermophilic (55.0 °C) and mesophilic (37.0 °C) ASBR reactor was dominated by Thermoanaerobacterium sp. based on the PCR-DGGE technique.
Keywords:Thermophilic-mesophilic system  Two-stage  Renewable energy  Mixed culture  POME  Microbial community analysis
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