Optimization of fermentative hydrogen production by Enterococcus faecium INET2 using response surface methodology |
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| Affiliation: | 1. Tsinghua University, Zhang Jiagang Joint Institute for Hydrogen Energy and Lithium-Ion Battery Technology, INET, Tsinghua University, Beijing 100084, PR China;2. Beijing Key Laboratory of Radioactive Waste Treatment, INET, Tsinghua University, Beijing 100084, PR China;1. Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing 100084, PR China;2. Beijing Key Laboratory of Radioactive Waste Treatment, Tsinghua University, Beijing 100084, PR China;1. Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India;2. Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea;1. Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing 100084, PR China;2. Beijing Key Laboratory of Radioactive Waste Treatment, INET, Tsinghua University, Beijing 100084, PR China;1. Department of Environmental Engineering and Science, Feng Chia University, Taichung 40724, Taiwan;2. Green Energy Development Center, Feng Chia University, Taichung 40724, Taiwan;3. Master Program of Green Energy Science and Technology, Feng Chia University, Taichung 40724, Taiwan |
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| Abstract: | A newly isolated strain Enterococcus faecium INET2 was used as inoculum for biohydrogen production through dark fermentation. The individual and interactive effect of initial pH, operation temperature, glucose concentration and inoculation amount on the accumulation of hydrogen during fermentation was examined by a Box–Behnken Design (BBD), and hydrogen production process was analyzed at the optimal condition. A significant interactive effect between glucose concentration and pH was observed, the optimal condition was initial pH 7.1, operation temperature 34.8 °C, glucose concentration 11.3 g/L and inoculation amount 10.4%. Hydrogen yield, maximum hydrogen production rate and hydrogen production potential were determined to be 1.29 mol H2/mol glucose, 86.7 L H2/L/h and 1.35 L H2/L. Metabolites analysis showed that E. faecium INET2 followed the pyruvate: formate lyase (Pfl) pathway in first 16 h, followed by the acetate-type fermentation and then shifted to butyrate-type fermentation. Maximum hydrogen production rate was accompanied with a quick formation of acetic acid. |
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| Keywords: | Biohydrogen Dark fermentation Optimization |
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