Dark and photofermentation H2 production from hydrolyzed biomass of the potent extracellular polysaccharides producing cyanobacterium Nostoc commune and intracellular polysaccharide (glycogen) enriched Anabaena variabilis NIES-2095 |
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| Affiliation: | 1. Biology Department, Faculty of Science, Taibah University, Medina, Saudi Arabia;2. Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, Egypt;3. Microbiology Department, Faculty of Agriculture, Fayoum University, Cairo, Egypt;4. Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt;5. Chemistry Department, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia;6. Chemistry Department, Faculty of Science, Assiut University, Assiut, Egypt;1. Department of Civil and Environmental Engineering, University of Rome “La Sapienza”, Italy;2. Department of Civil and Environmental Engineering and Architecture, University of Cagliari, Italy;3. IGAG – CNR (Environmental Geology and Geoengineering Institute of the National Research Council), Italy;1. Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan;2. Department of Chemical Engineering, National Taiwan University, Taipei 10617 Taiwan;3. Department of Chemical and Materials Engineering, Tunghai University, Taichung 407, Taiwan;4. Center for Nanotechnology, Tunghai University, Taichung 407, Taiwan;5. Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, 10607 Taiwan;1. Collaborative Innovation Center of Biomass Energy, Henan Agricultural University, Zhengzhou 450002, China;2. Master''s Program of Green Energy Science and Technology, Feng Chia University, Taichung 40724, Taiwan;3. Quasar Energy Group, 2705 Selby Rd., Wooster, OH 44691, United States;1. Henan Agricultural University, Henan International Joint Laboratory of Biomass Energy and Nanomaterials, Zhengzhou 450002, PR China;2. Huanghe S & T University, Zhengzhou 450006, China;1. Biology Department, Faculty of Science, Taibah University, Medina, Saudi Arabia;2. Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, Egypt |
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| Abstract: | The biological H2 production industry would be independent from other industries if it has its own supply of organic materials especially in non-agricultural countries. In this study, acid hydrolyzed biomass of the potent extracellular polysaccharides (EPSs) producing cyanobacterium Nostoc commune and glycogen (as intracellular polysaccharide) enriched Anabaena variabilis NIES-2095 were used as a cheap organic carbon feedstock for biological H2 production by two stages dark fermentation by Escherichia coli strain MWW and Clostridium acetobutylicum DSM-792 or Clostridium beijerinckii DSM-1820 and photofermentation by Rhodobacter capsulatus JCM-21090 under anaerobic conditions. Acid hydrolysis of air dried cyanobacterial biomass was conducted at optimum conditions of 4 M HCl at 120 °C in an autoclave for 30 min and subsequently neutralized and used as an organic carbon source for first stage dark fermentation followed by a second stage photofermentation. The facultative anaerobe Escherichia coli strain MWW was used for maintaining anaerobiosis. Escherichia coli strain MWW was isolated and identified by morphological and biochemical characterizations as well as molecular biological phylogenetic analysis of its 16S rDNA sequence. Nostoc commune was identified by morphological and microscopic characterizations and by 16S rDNA sequence phylogenetic analysis. The two stages dark fermentation by Escherichia coli and Clostridium acetobutylicum or Clostridium beijerinckii and photofermentation by Rhodobacter capsulatus produced in total 5.9 and 5.6 mol H2/mole reducing sugars of acid hydrolyzed Nostoc commune EPSs/biomass, respectively and 5.43 and 5 mol H2/mole reducing sugars of acid hydrolyzed biomass of glycogen enriched Anabaena variabilis, respectively. These results indicate a high potency of using cyanobacterial polysaccharides/biomass (extracellular polysaccharides and intracellular glycogen) as an organic carbon source for H2 production which would be of importance for non-agricultural countries. |
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| Keywords: | Biohydrogen Cyanobacterial polysaccharides |
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