High-performance liquid chromatography of human milk triacylglycerols and gas chromatography of component fatty acids |
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Authors: | Karen D. Dotson John P. Jerrell Mary Frances Picciano Edward G. Perkins |
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Affiliation: | (1) Division of Nutritional Sciences, University of Illinois, Burnsides Research Laboratory, 1208 Pennsylvania Avenue, 61801 Urbana, IL;(2) Department of Food Sciences, University of Illinois Burnsides Research Laboratory, 1208 Pennsylvania Avenue, 61801 Urbana, IL;(3) Department of Nutrition, Pennsylvania State University, 16802 University Park, Pennsylvania |
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Abstract: | Human milk triacylglycerols were separated by high-performance liquid chromatography. A 5-μ Supelcosil LC-18 column (Supelco, Inc., Bellefonte, PA) was used with acetone/acetonitrile (64∶36, vol/vol) as mobile phase. Triacylglycerols were tentatively identified based on theoretical carbon number and relative retention time. Despite changes resulting from dietary fat variation, the major component triacylglycerols were those composed of palmitic, oleic and linoleic acids. Triacylglycerols with palmitic, stearic and oleic acids were present as minor components. Fatty acids were quantified by gas chromatography relative to an internal standard. Ratios of n−6/n−3 fatty acids were found to be high than previously reported. Based on a paper presented at the Symposium on Milk Lipids held at the AOCS Annual Meeting, Baltimore, MD, April 1990. |
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