首页 | 本学科首页   官方微博 | 高级检索  
     


13-Hydroxy-9Z,15Z-Octadecadienoic Acid Production by Recombinant Cells Expressing Lactobacillus acidophilus 13-Hydratase
Authors:Woo-Ri Kang  Chul-Soon Park  Kyung-Chul Shin  Kyoung-Rok Kim  Deok-Kun Oh
Affiliation:, Department of Bioscience and Biotechnology, Konkuk University, Seoul, South Korea
Abstract:Recombinant Escherichia coli cells expressing linoleate 13‐hydratase from Lactobacillus acidophilus were permeabilized by treating with 0.2 M NaCl. The optimal conditions for the production of 13‐hydroxy‐9,15(Z,Z)‐octadecadienoic acid (13‐HODE) from α‐linolenic acid by permeabilized recombinant cells were pH 6.0, 40 °C, 7.5 % (v/v) methanol, 60 g/l permeabilized cells, and 15 g/l α‐linolenic acid. Under these conditions, permeabilized cells produced 7.5 g/l 13‐HODE after 6 h, with a conversion yield of 50 % (w/w) and a volumetric productivity of 1.25 g/l/h. These values were 161 and 160 % of those obtained by nonpermeabilized cells, respectively. To the best of our knowledge, this is the first report on the process optimization for the biotechnological production of 13‐HODE.
Keywords:Cell permeabilization  Lactobacillus acidophilus  13‐Hydroxy‐9,15(Z,Z)‐octadecadienoic acid  Linoleate 13‐hydratase  α‐Linolenic acid
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号