北虫草醇提物体外抗肿瘤和抗病毒活性的比较研究

采用体外人肠腺癌细胞株SW620和人乳腺癌细胞株MCF-7的增殖抑制试验模型和抗病毒(抗流感甲型病毒和抗疱疹病毒)的筛选模型,对北虫草[Cordyceps militaris(L.ex)Link]七个菌株(CM1-CM7)的菌丝体醇提物(CME1-CME7)的抗肿瘤、抗病毒活性进行了比较研究。抗肿瘤试验表明,在中、高浓度时(50μg/mL、200μg/mL,七个醇提物CME1~CME7对SW620和MCF-7的增殖均有一定的抑制活性,其中CME7活性最高(66.0±2.3)%,与阳性对照5-Fu(69.1±4.5)%抑制活性相当。抗病毒试验表明,七个菌株的醇提物中,仅CME7和CME6具有抗流感甲型病毒活性,半数有毒浓度(TC50)分别为288.68μg/mL和>500μg/mL,选择指数(SI)分别为2.23和>3.87;七个菌株的醇提物均未表现出对疱疹病毒HSV-Ⅰ和HSV-Ⅱ的抗性。因此,在七个菌株中,CME7的抗肿瘤活性和抗病毒活性均较好,有必要对其进一步研究研究和开发。

Comparative Study on the Antitumor Effect and Antiviral Activity of Ethanol Extracts from Codyceps militaris Mycelia in Vitro

In vitro,the antitumor effects of ethanol extracts from Codyceps militaris mycelia were investigated by using proliferation-inhibition for tumor cells(SW620 and MCF-7),and the antiviral activity of ethanol extracts were assessed by using screening model on viruses A-FLU,HSV-Ⅰand HSV-Ⅱ.The antitumor results showed that seven samples(CME1-CME7) had evident antitumor activity on tumor cells at concentrations of 50 μg/mL and 200 μg/mL,and CME7 was the best one among all samples,and at 200 μg/mL concentration,exhibited a similar potency to 1.0 μg/mL 5-Fu,which served as the positive control.The antiviral activity assay showed that the both samples CME7 and CME6,had the best antiviral activity on the virus A-FLU among three strains,and their TC50 were 288.68 μg/mL and >500 μg/mL,respectively,and their SI were 2.23 and >3.87,respectively.Seven strains had hardly any inhibitive effect on virus HSV-Ⅰand HSV-Ⅱ.Therefore,conclude that the strain CM7 was the best one among seven strains,and it is potential resources to be used as bioactive natural agents.