| SBA生物传感仪酶法测定谷氨酸定标值的校正 |
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| 引用本文： | 张宏建,段作营,毛忠贵. SBA生物传感仪酶法测定谷氨酸定标值的校正[J]. 食品与生物技术学报, 2002, 21(5): 529-532 |
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| 作者姓名： | 张宏建 段作营 毛忠贵 |
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| 作者单位： | 江南大学,工业生物技术教育部重点实验室,江苏,无锡,214036 |
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| 摘 要： | 在味精行业中 ,目前广泛采用SBA生物传感仪检测L 谷氨酸含量 .SBA生物传感仪在检测量程下 ,定标为 10 0 ,但在检测谷氨酸发酵液、等电上清液、上清液的浓缩液、脱盐液以及脱色液时 ,受NH4 +的影响 ,其结果偏低 ;为此 ,其定标必须进行相应的调整 ,才能准确地检测出样品中的谷氨酸含量 .
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| 关 键 词： | SBA生物传感仪 谷氨酸 铵离子 |
| 文章编号： | 1009-038X(2002)05-0529-04 |
| 修稿时间： | 2002-04-18 |
The Emendation of Calibrating Value of Glutamic Acid Detection Using Enzymatic Method with SBA-Biosensor |
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| ZHANG Hong jian,DUAN Zuo ying,MAO Zhong gui. The Emendation of Calibrating Value of Glutamic Acid Detection Using Enzymatic Method with SBA-Biosensor[J]. Journal of Food Science and Biotechnology, 2002, 21(5): 529-532 |
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| Authors: | ZHANG Hong jian DUAN Zuo ying MAO Zhong gui |
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| Abstract: | SBA biosensor was widely used for measuring the content of L glutamic acid in monosodium glutamate production nowadays. Under the detection range, the standard solution was set 100. But when we detected the fermenting liquor, the upper liquid after equipotential point, the upper liquid of concentrates, and the desalting and decoloring liquor, the detecting system was easily interfered by ammonia and it's result was on the low side. Thus the standard value must be adjusted accordingly, and the L glutamic acid contents of fermenting liquor then could be exactly detected. |
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| Keywords: | SBA biosensor glutamic acid ammonia |
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