| 梅毒螺旋体血凝试剂盒的研制 |
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| 引用本文： | 张昆,范亚民,王薇媛,沈荣,李安敏,周学良,辜清吾. 梅毒螺旋体血凝试剂盒的研制[J]. 中国生物制品学杂志, 1991, 0(1) |
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| 作者姓名： | 张昆 范亚民 王薇媛 沈荣 李安敏 周学良 辜清吾 |
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| 作者单位： | 新疆地方病防治研究所，卫生部兰州生物制品研究所，中国药品生物制品检定所，卫生部兰州生物制品研究所，卫生部兰州生物制品研究所，中国药品生物制品检定所 乌鲁木齐 830002 |
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| 摘 要： | 以超声波处理的梅毒螺旋体为抗原,致敏经醛化、鞣化处理的绵羊红血球,应用血凝试验检测梅毒患者血清中梅毒螺旋体特异性抗体。同时以国际上公认的标准方法——FTA-ABS作为对照试验。共检测1125份血清(其中梅毒血清205份,非梅毒血清920份).两种方法检测结果完全相符,即敏感性皆为100％,血凝试验的特异性为99％。
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| 关 键 词： | 梅毒密螺旋体 梅毒螺旋体 血凝试验 |
Developnent of the Treponema Pallidum Hemagglutination Test Kit |
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| Zhang Kun et al Xinjiang Institute for Endemic Disease Control and Research. Developnent of the Treponema Pallidum Hemagglutination Test Kit[J]. Chinese Journal of Bilogicals, 1991, 0(1) |
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| Authors: | Zhang Kun et al Xinjiang Institute for Endemic Disease Control and Research |
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| Affiliation: | Zhang Kun et al Xinjiang Institute for Endemic Disease Control and Research |
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| Abstract: | The Treponema pallidum hemaggl utination test (TPHA) kit for syphilis using sonicated, purified T. pallidum (Nichols strain) antigen coupled to glutaraldehyde-stabilized sheep erythrocyte was prepared and compared with FTA-ABS and TP-ELISA. A total of 205 cases of syphilis was studied with 100% agreement between the TPHA and FTA-ABS. This kit was also tested with 920 non-syphilis sera, the false-positive rate was 1.0% for TPHA, and no false-positive for FTA-ABS.In conclusion, the TPHA was comparable in sensitivity and specificity to FTAABS test for syphilis and non-syphilis sera. Compared with the TP-ELISA and FTAABS, the TPHA was more easier and economical to perform. |
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| Keywords: | T. pallidum TPHA |
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