Competitive binding assay for glucose based on glycodendrimer-fluorophore conjugates |
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Authors: | Ibey Bennett L Beier Hope T Rounds Rebecca M Coté Gerard L Yadavalli Vamsi K Pishko Michael V |
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Affiliation: | Department of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, USA. bli6339@tamu.edu |
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Abstract: | A new fluorescent glucose assay has been created using Alexa Fluor 647-labeled concanavalin A (Con A) and a fourth-generation PAMAM Alexa Fluor 594-labeled glycodendrimer. This assay has been shown to have a large response to glucose within the biological range and to be capable of functioning within a polymer hydrogel. In this paper, the glucose response is shown to be a single fluorophore-based quenching reaction. Data showing that the sensor is fully reversible and specific through competitive binding between the dendrimer and glucose with Con A are presented. Overall, the assay is shown to have potential over the traditional dextran-based assay because it has a larger dynamic response to physiological glucose concentrations, incorporates longer wavelength dyes that improve signal penetration through dermal tissue, and provides an internal reference in the form of a nonreactive fluorescent label. |
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