| 偶氮胭脂红G结合共振光散射法测定人血清样品中的总蛋白 |
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| 引用本文： | 许立松,王颖臻,曹秋娥,丁中涛.偶氮胭脂红G结合共振光散射法测定人血清样品中的总蛋白[J].云南化工,2009,36(3):79-82. |
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| 作者姓名： | 许立松 王颖臻 曹秋娥 丁中涛 |
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| 作者单位： | 1. 昆明学院,云南,昆明,650031
2. 教育部自然资源药物化学重点实验室,云南大学化学科学与工程学院,云南,昆明,650091 |
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| 摘 要： | 研究了在pH为2.5的BR缓冲酸性介质中,偶氮胭脂红G与牛血清白蛋白(BSA)、人血清白蛋白(HSA)、鸡蛋白蛋白(OVA)及免疫球蛋白(γ-IgG)等蛋白质作用产生共振光散射(RLS)增强信号,最大散射峰位于594nm处。在最佳实验条件下,增强RLS强度在594 nm处与蛋白质浓度呈线性关系,检出限在0.025~0.406μg/mL之间,生物体液中大部分常见物质均不产生干扰。该法应用于人血清样品测定,测定结果与考马斯亮蓝法一致。
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| 关 键 词： | 共振光散射 偶氮胭脂红G 蛋白质 |
Determination of Total Protein from Human Blood Plasma by Resonance Light Scattering Technique with AzocarmineG |
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| Xu Li-song,Wang Ying-zhen,Cao Qiu-e,Ding Zhong-tao.Determination of Total Protein from Human Blood Plasma by Resonance Light Scattering Technique with AzocarmineG[J].Yunnan Chemical Technology,2009,36(3):79-82. |
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| Authors: | Xu Li-song Wang Ying-zhen Cao Qiu-e Ding Zhong-tao |
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| Affiliation: | Xu Li-song ,Wang Ying-zhen , Cao Qiu-e ,Ding Zhong-tao ( 1. Department of Chemical Science and Technology, Kunming School, Kunming 650031 ; 2. Key laboratory of Medicinal Chemistry for Nature Resource, Ministry of Education, School of Chemical Science and Technology, Yunnan University, Kunming 650091 ) |
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| Abstract: | Enhanced resonance light scattering signal （RLS） at 594 nm was studied by interactions of azoarmine G with bovine serum albumin, human serum albumin and 7 - globulin, in acidic medium of pH 2.5. Under optimal condition, the intensity of enhanced RLS was linear proportional to the concentration of proteins, with the detective limit of 0. 025 - 0.406μ/ml and no disturbance of most common substances. The method was successfully applied to the determination of protein from human blood plasma sample, consistent with that by Coomassie brilliant blue G250 assay. |
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| Keywords: | resonance light scattering azocarmine G protein |
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