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| 水-有机溶剂混合体系脂包衣酶制备S-(+)-布洛芬 | |
| 引用本文： | 杨根生,吴伟军,欧志敏,姚善泾.水-有机溶剂混合体系脂包衣酶制备S-(+)-布洛芬[J].化学工程,2010,38(1). |
| 作者姓名： | 杨根生 吴伟军 欧志敏 姚善泾 |
| 作者单位： | 1. 浙江大学,化学和生物工程系,浙江,杭州,310027;浙江工业大学,药学院,浙江,杭州,310032 2. 浙江工业大学,药学院,浙江,杭州,310032 3. 浙江大学,化学和生物工程系,浙江,杭州,310027 |
| 基金项目： | 浙江省自然科学基金资助项目(Y404353) |
| 摘 要： | 用二-十二烷基-N-D-葡萄糖酸-L-谷氨酸酯对来源于Candida cylindrucea的脂肪酶包衣后应用于水-有机溶剂体系中催化选择性水解布洛芬甲酯制备S-(+)-布洛芬。考察了体系的有机溶剂种类和体积分数、温度、pH值等因素对转化率和对映选择性的影响。结果表明,在布洛芬甲酯为100 mmol,脂包衣脂肪酶250 mg/mL,pH值为7.5,温度为40℃,N,N-二甲基甲酰胺(DMF)体积分数在20%时,15 h后催化的转化率可达48.3%,对映选择率达465。动力学研究表明脂包衣脂肪酶在水-DMF混合体系中其酶促反应符合米氏方程,其米氏常数Km是水相中没有包衣酶的1/2,最大反应速度vm ax是它的1.4倍。 |
| 关 键 词： | 脂包衣脂肪酶 水-有机溶剂混溶 布洛芬 拆分 |
Preparation of S-(+)-ibuprofen by lipid-coated enzymes in water-miscible organic-solvents |
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| YANG Gen-sheng,WU Wei-jun,OU Zhi-min,YAO Shan-jing.Preparation of S-(+)-ibuprofen by lipid-coated enzymes in water-miscible organic-solvents[J].Chemical Engineering,2010,38(1). | |
| Authors: | YANG Gen-sheng WU Wei-jun OU Zhi-min YAO Shan-jing |
| Affiliation: | 1.Department of Chemical and Biochemical Engineering;Zhejiang University;Hangzhou 310027;Zhejiang Province;China;2.College of Pharmaceutical Sciences;Zhejiang University of Technology;Hangzhou 310032;China |
| Abstract: | The catalytic activity of enantioselective hydrolysis of ibuprofen methyl ester by the Candida cylindrucea lipase coated with didodecyl N-D-glucono-L-glutamate in the water-miscible organic-solvent system was studied.The main variables affecting the conversion rate and the enantiomeric selectivity by the lipid-coated lipase were optimized,including kinds and volume fraction,temperature,pH of organic solvent.The results show that the total conversion rate and the enantiomeric ratio can reach 48.3% and 465,re... |
| Keywords: | lipid-coated lipase water-miscible organic-solvent ibuprofen resolution |
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