染料木黄酮钙配合物的合成、表征及清除自由基和抑菌活性

以染料木黄酮和氢氧化钙为原料合成了染料木黄酮钙配合物,并采用红外光谱、紫外光谱、热重分析及核磁共振等手段对其结构进行了表征。采用邻苯三酚自氧化法、Fenton反应-邻二氮菲光度法和DPPH自由基方法分别测定配体与配合物清除超氧自由基、羟基自由基和DPPH自由基的能力,并且考察了它们的抑菌性质,测定了其抑制大肠杆菌、绿脓杆菌、李斯特氏菌的能力。结果表明:染料木黄酮以分子中的4位羰基O和5位羟基O与Ca2+配位而形成分子式为Ca(C15H9O5)2].2H2O的配合物。在相同浓度下,配合物清除超氧自由基和羟自由基的能力明显强于配体,而清除DPPH自由基的能力仅略有增强。配体及其配合物对大肠杆菌、李斯特氏菌、绿脓杆菌都有一定的抑菌效果,配合物的抑菌性仅略高于配体。

Synthesis, characterization, scavenging free radical and bacteriostasis characteristics of calcium-genistein complex

By using genistein and calcium hydroxide as raw materials, calcium-genistein complex was synthesized and characterized with IR spectra, UV-spectra, thermal analysis and NMR spectrum. The biological activities of the complex were studied by means of pyrogallol autoxidation, Fenton reaction, orthophenanthroline photometry and DPPH free radical. And also the bacteriostasis activities of the ligand and its complex against three different types of bacteria, namely: Escherichia coli，Pseudomonas aeruginosa，Listeria monocytogenes were studied.  The results showed that in calcium-genistein complex Ca²⁺ was coordinated by 5-OH and 4-CO groups of genistein and the molecular formula was［Ca(C₁₅H₉O₅)₂］·2H₂O. At the same concentration, the scavenging effects of Gen-Ca on superoxide radical and anti-hydroxy free radical showed better activity than the free ligand, but anti-DPPH free radical ability was not so obvious. The ligand and its complex had certain bacteriostasis effect, and the complex showed increased activity compared to the respective ligand.