正相高效液相色谱法测定花生油中的维生素E组分

建立正相高效液相色谱法测定花生油中的维生素E组分的方法。花生油中维生素E用正己烷直接提取后采用正相高效液相色谱法,荧光检测器检测。维生素E各组分浓度在0.01~100g/m L峰面积与含量具有良好的线性关系,平均相对标准偏差在0.47%~0.92%,平均回收率为94.9%~98.7%。该方法快速,准确,灵敏度高,可作为花生油中维生素E含量测定的检测方法。

Determination of Vitamin E Components in Peanut Oil by Normal-phase High Performance Liquid Chromatography

A normal-phase high performance liquid chromatography method was developed for determination of vitamin E components in peanut oil. Vitamin E components in peanut oil samples were extracted by n-hexane, and then determined by normal-phase high performance liquid chromatography with fluorescence detection. Results showed that0.01~100 g/m L vitamin E components and peak areas were of good linear relationship. Average relative standard deviations were 0.47%~0.92% and average spike recoveries were 94.9%~98.7%. This method demonstrated to be simple and accurate with high precision, and thus had a promising potential for the determination of vitamin E in peanut oil.