Biosensor enhanced by glucose oxidase biomimetic membrane containing the platinum and silica nanoparticles |
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Authors: | Xiangling Ren Xianwei Meng Fangqiong Tang Lin Zhang |
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Affiliation: | aLaboratory of Controllable Preparation and Application of Nanomaterials, Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry of Chinese Academy of Sciences, Beijing 100190, PR China |
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Abstract: | In this paper, glucose biosensor is fabricated with immobilization of glucose oxidase (GOx) in platinum and silica sol. The glucose biosensor combined with Pt and SiO2 nanoparticles could make full use of the properties of nanoparticles. A set of experimental results indicates that the current response for the enzyme electrode containing platinum and silica nanoparticles increases from 0.32 µA cm− 2 to 33 µA cm− 2 in the solution of 10 mM β-D-glucose. The linear range is 3 × 10− 5 to 3.8 × 10− 3 M with a detection limit of 2 × 10− 5 M at 3σ. The effects of the various volume ratios of Pt and SiO2 sols with respect to the current response and the stability of the enzyme electrodes are studied. |
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Keywords: | Platinum nanoparticles Silica nanoparticles Immobilization enzyme Glucose oxidase Biosensor |
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