Characterization of morphine-specific monoclonal antibodies showing minimal cross-reactivity with codeine |
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Authors: | T Usagawa Y Itoh E Hifumi A Takeyasu Y Nakahara T Uda |
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Affiliation: | Ube Laboratory, Ube Industries, Ltd., Japan. |
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Abstract: | Six murine monoclonal antibodies against morphine were produced using N-(4-aminobutyl)normorphine as a hapten. Most of the antibodies obtained distinguished the substituents at the 3 and 6 positions of morphine. This property of the antibodies led to a reduction in cross-reactivity with codeine, morphine-3-glucuronide (M-3-G) and morphine-6-glucuronide (M-6-G) to negligible levels. However, one of the antibodies distinguished the substituent only at the 3 position of morphine, which cross-reacted with M-6-G, naloxone and naltrexone. In the competitive inhibition enzyme-linked immunosorbent assay, morphine was detected at concentrations as low as circa 100 pg/ml. |
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