Purification and characterization of deep sea sharkCentrophorus squamosus liver oil 1-O-aklylglycerol ether lipids |
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Authors: | Catherine G Bordier Nicole Sellier Alain P Foucault François Le Goffic |
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Affiliation: | (1) Laboratoire de Bioorganique et Biotechnologies, ENSCRP-CNRS, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France;(2) Laboratoire de Spectrométrie de Masse, ENSCP-CNRS, 75231 Paris Cedex 05, France |
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Abstract: | The 1-O-alkylglycerol composition of the liver oil of the deep sea sharkCentrophorus squamosus, a species which provides edible flesh, has been determined. After various fractionations of the oil, the unsaponifiable
fraction was characterized by means of gas chromatography/mass spectrometry, electron impact, and positive-ion chemical ionization.
The oil is composed of 60% unsaponifiable matter, containing 45% squalene, 4.5% cholesterol, and 10% of linear saturated and
monounsaturated glycerol ethers with 14–18 carbon atoms. After a first separtion by chromatography on silicic acid, monounsaturated
glycerol ethers have been separated from the saturated homologues, in particular from 1-O-octadecylglycerol (batyl alcohol) and 1-O-hexadecylglycerol (chimyl alcohol)via urea complexation. This newer application of the urea method, already used in the past to extract saturated from polyunsaturated
fatty acids, allowed the purification of the main components of the complex unsaturated glycerol ether fraction, namely, 1-O-octadecen-9′ylglycerol (selachyl alcohol) and 1-O-hexadecen-9′ylglycerol. |
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