金银花中绿原酸的分离纯化工艺研究

目的:对金银花中的绿原酸的提取、分离工艺进行研究。方法:绿原酸经乙醇热回流提取后,采用聚酰胺柱层析法进行分离除杂,对主要工艺参数进行了优化。结果:将待分离的绿原酸粗品和聚酰胺(80～100目)按物料比1:5装入柱径与柱长比为1:7的层析柱中,常压下用10%的乙醇以最大流速洗脱至14倍柱体积,收集绿原酸含量较高(A322/A216≥0.9)的流分,浓缩后得到纯度为70%的绿原酸产品,经重结晶后纯度可提高到93%。结论:该工艺简单、成本较低,适于工业化。

Study on Isolation and Purification of Chlorogenic Acid in Flos Lonicerae

Objecive: To study the isolation and purification conditions of chlorogenic acid(CA) from Flos Lonicerae. Method: The isolation and purification of CA were achieved by polyamide column chromatography and the main isolation factors were optimized. Results: The optimum isolation is as following: the rate of crude CA to polymide is 1:5, the rate of diameter to long is 1:7, 10% alcohol washed at high speed under usual pressure, CA is collected between the volume of 5 ～ 14 times of column volume(A322/A216 ≥0.9), under the conditions, the product with above 70% CA is obtained, which can be further crystalled by EtoAc and the purity is enhanced to 93%. Conclusion: This method is simple, low cost, and suitable to be used in industry.