| 微柱高效液相色谱法测定烟草样品中植物色素的研究 |
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| 引用本文： | 吴方评,董学畅,张甜,杨光宇. 微柱高效液相色谱法测定烟草样品中植物色素的研究[J]. 云南化工, 2005, 32(1): 24-27 |
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| 作者姓名： | 吴方评 董学畅 张甜 杨光宇 |
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| 作者单位： | 1. 云南民族大学,化学与生物技术学院,云南昆明,650031
2. 云南省烟草科学研究院,云南昆明,650106 |
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| 基金项目： | 国家烟草专卖局科研基金资助课题(2001 B205) |
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| 摘 要： | 通过微柱高效液相色谱法测定烟草样品中的植物色素;用 90%丙酮震荡浸提烟草样品中的色素,提取液经WatersSep Park-C18固相萃取小柱预分离和富集,以WatersXterraTMRP18 (1. 0×50mm, 2. 5μm)色谱柱为固定相, (1+1)甲醇异丙醇溶液-水梯度为流动相,烟叶中的叶绿素 a、叶绿素 b、β 胡萝卜素、叶黄素、新叶黄素、紫黄质和脱镁叶绿素等色素分别在其最大吸收波长监测下分离提取,色谱峰面积定量。该方法标准回收率95% ~105%, RSD为 1. 6% ~2. 5%。将其用于烟草中天然色素含量的测定,结果令人满意。
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| 关 键 词： | 微柱高效液相色谱法 固相萃取 植物色素 烟叶 |
| 文章编号： | 1004-275X(2005)01-0024-04 |
| 修稿时间： | 2005-01-05 |
Determination of Pigments from Tobacco Leaf by Microcolumn High Performance Liquid Chromatography |
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| WU Fang-ping,DONG Xue-chang,ZHANG Tiang,YANG Guang-yu. Determination of Pigments from Tobacco Leaf by Microcolumn High Performance Liquid Chromatography[J]. Yunnan Chemical Technology, 2005, 32(1): 24-27 |
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| Authors: | WU Fang-ping DONG Xue-chang ZHANG Tiang YANG Guang-yu |
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| Affiliation: | WU Fang-ping,DONG Xue-chang~1,ZHANG Tiang1,YANgG Guang-yu ~2 |
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| Abstract: | Determination of pigments from tobacco leaf was studied by microcolumn high performance liquid chromatography. The tobacco leaf pigments was extracted from tobacco leaf with 90% acetone and then purified and enriched by solid phase extraction with Sep-Pak-C18 cartridge. Chlorophylls-a and b, (-carotene, lutin, neoxanthin, violaxanthin, pheophorbide and other tobacco leaf pigments could be separated and determinated on a Waters Xterra TM RP18 (1.0×50 mm, 2.5 μm) microcolumn, eluting with methanol-isopropyl alcohol-water gradient (1+1). Recovery of tobacco leaf pigments were 95%~105%, with relative standard deviation of 1.6~2.5%. The method could be applied to determine pigments from tobacco leaf with satisfactory result. |
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| Keywords: | microcolumn high performance liquid chromatography solid phase extraction tobacco leaf pigments tobacco leaf |
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