引进美国烤烟新品种(系)的抗性检测与评价

为选育和利用抗病品种、有效防治烟草病害,采用人工诱发抗性鉴定方法对引进的13个美国烤烟品种(系)进行了烟草黑胫病、烟草普通花叶病(TMV)和赤星病的抗性测定,并采用分子标记方法对黑胫病和TMV进行了辅助检测。结果表明:NCT5、NCT6、NCT7、NCT8、NCT9、NCT10和NCT13抗黑胫病,NCT1、NCT4、NCT11和NCT12中抗黑胫病,抗病分子标记辅助检测中仅有4个烤烟品种NCT6、NCT9、NCT12和NCT13检测出Ph基因,其余品种未检出含有Ph基因。NCT1、NCT8、NCT11和NCT4中感赤星病,NCT12、NCT10、NCT2、NCT13、NCT9、NCT3、NCT7、NCT6和NCT5中抗赤星病。NCT13、NCT10、NCT9、NCT12、NCT6、NCT4、NCT1、NCT2、NCT11、NCT7和NCT8感TMV,NCT5和NCT3中感TMV。PCR分析表明,所有供试品种(系)均不含N基因,与抗性鉴定结果中13个供试品种(系)对TMV均表现感病和中感的结果相吻合。 

Testing and Evaluation of Disease Resistance of Flue-cured Tobacco Varieties(Lines) Introduced From U.S.

For breeding resistant varieties,the resistances of 13 flue-cured tobacco varieties(lines) introduced from the U.S.to tobacco black shank,TMV and tobacco brown spot diseases were tested with inoculation,and the tests to TMV and brown spot diseases were assisted with molecule marking.The results indicated that:1) NCT5,NCT6,NCT7,NCT8,NCT9,NCT10 and NCT13 were resistant,NCT1,NCT4,NCT11 and NCT12 were moderately resistant to tobacco black shank.In molecule marking testing,the dominant black shank resistance gene,Ph,was detectable only in NCT6,NCT9,NCT12 and NCT13.2) To tobacco brown spot disease,NCT1,NCT8,NCT11 and NCT4 were moderately susceptible,NCT12,NCT10,NCT2,NCT13,NCT9,NCT3,NCT7,NCT6 and NCT5 were moderately resistant.3) To TMV disease,NCT13,NCT10,NCT9,NCT12,NCT6,NCT4,NCT1,NCT2,NCT11,NCT7 and NCT8 were susceptible,NCT5 and NCT3 were moderately susceptible.PCR analysis results showed that TMV resistance gene,N,was not detectable in all varieties(lines) mentioned above,which agreed with resistance evaluation results.