不同抽吸模式下烟气冷凝物对人口腔细胞体外生长增殖的影响

为考察卷烟烟气暴露对人口腔细胞体外生长增殖的影响,以肯塔基参比卷烟3R4F 为试验卷烟,人口腔表皮样癌细胞为实验模型,分别在ISO 和加拿大深度抽吸(HCI)模式下制备烟气冷凝物并对细胞进行染毒暴露,评估了两种抽吸模式下烟气暴露对人口腔细胞代谢活性及核酸复制活性的影响,并结合主流烟气总粒相物(TPM)中主要有害成分的释放水平进行了分析。结果表明:①烟气冷凝物暴露对人口腔细胞体外增殖有抑制作用,对人口腔细胞代谢活性以及核酸的复制活性有一定的毒性作用。②HCI 抽吸模式下3R4F 卷烟单位TPM 和单位烟碱中有害成分的释放量低于ISO 模式,HCI 抽吸模式下制备的烟气冷凝物含水率高于ISO 模式,这两种因素是导致3R4F 卷烟HCI 模式下烟气冷凝物细胞毒性弱于ISO模式的主要原因。 

Effects of Cigarette Smoke Condensate Collected Under Different Smoking Regimes on in vitro Proliferation of Human Oral Cell

In order to investigate the effects of exposure to cigarette smoke on the in vitro proliferation of human oral cell, Kentucky 3R4F reference cigarette was used as experimental cigarette and human oral epidermoid carcinoma cell line KB as experimental model. Cigarette smoke condensates (CSCs) were collected under ISO and Health Canada Intense (HCI) smoking regimes for the exposure assessment of human oral cells, the effects of CSCs on the metabolic activity and nucleic acid replication activity of the cells were assessed, and further analyzed versus the release levels of main harmful components in total particulate matter (TPM) of mainstream cigarette smoke. The results indicated that: 1) CSCs exposure inhibited the in vitro proliferation of oral cells and imparted certain toxicity to their metabolic activity and nucleic acid replication activity. 2)Under HCI smoking regime, the release of harmful components per unit TPM and per unit nicotine in mainstream smoke of 3R4F reference cigarette was lower and the moisture content in CSCs was higher than those under ISO regime; these two factors explained the lower cytotoxicity of HCI regime CSCs.