烟草NtMAPK基因克隆及激素诱导条件下的表达分析

促分裂素原活化蛋白激酶(Mitogen-activated protein kinase,MAPK)是植物体防御反应信号物质中的关键组分和植物应答逆境胁迫的重要信号分子。为了探索MAPK基因同源体在烟草中的功能,采用RT-PCR技术从烟草中克隆出了一个MAPK同源序列,命名为NtMAPK。经氨基酸序列比对分析鉴定为栽培烟草MAPK家族的成员。系统发育分析显示,NtMAPK与水稻的OsMPK2/OsMPK62进化关系最近,表明NtMAPK可能具有与OsMPK62相似的功能,与植物体的抗病性相关。采用植物体防御反应相关的信号物质诱导,半定量分析结果显示在脱落酸(ABA)、茉莉酸甲酯(Me JA)和水杨酸(SA)诱导条件下该基因表达上调。 

Cloning of NtMAPK Gene in Tobacco and Analysis on its Expression Induced by Plant Hormone

Mitogen-activated protein kinase(MAPK) is a key component in signal substance in plant's defensive reaction and an important signal molecule in response to abiotic and biotic stresses. To reveal the function of its homolog genes in tobacco, a homolog sequence of MAPK was cloned from Nicotiana tabacum and named as NtMAPK. Amino acid sequence alignment analysis showed that NtMAPK gene belonged to MAPK family of cultivated tobacco.Phylogenetic analysis indicated that NtMAPK featured close phylogenetic relationship with rice OsMPK2/OsMPK62, it suggested that it might function similarly to OsMPK62 and related to plant's resistance to pathogens. Under the induction of plant defensive reaction-related signals, semi-quantitative PCR analysis revealed that the expression of NtMAPK was up-regulated by ABA, Me JA and SA.