Hydrogen production from soft-drink wastewater in an upflow anaerobic packed-bed reactor |
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Authors: | Guilherme PeixotoNora Katia Saavedra Maria Bernadete A. VarescheMarcelo Zaiat |
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Affiliation: | Laboratório de Processos Biológicos (LPB), Departamento de Hidráulica e Saneamento, Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Engenharia Ambiental - Bloco 4-F, Av. João Dagnone, 1100 - Santa Angelina, 13563-120 São Carlos, SP, Brazil |
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Abstract: | The production of hydrogen from soft-drink wastewater in two upflow anaerobic packed-bed reactors was evaluated. The results show that soft-drink wastewater is a good source for hydrogen generation. Data from both reactors indicate that the reactor without medium containing macro- and micronutrients (R2) provided a higher hydrogen yield (3.5 mol H2 mol−1 of sucrose) as compared to the reactor (R1) with a nutrient-containing medium (3.3 mol H2 mol−1 of sucrose). Reactor R2 continuously produced hydrogen, whereas reactor R1 exhibited a short period of production and produced lower amounts of hydrogen. Better hydrogen production rates and percentages of biogas were also observed for reactor R2, which produced 0.4 L h−1 L−1 and 15.8% of H2, compared to reactor R1, which produced 0.2 L h−1 L−1 and 2.6% of H2. The difference in performance between the reactors was likely due to changes in the metabolic pathway for hydrogen production and decreases in bed porosity as a result of excessive biomass growth in reactor R1. Molecular biological analyses of samples from reactors R1 and R2 indicated the presence of several microorganisms, including Clostridium (91% similarity), Enterobacter (93% similarity) and Klebsiella (97% similarity). |
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Keywords: | Low-density polyethylene Clostridium Enterobacter Packed-bed reactor |
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