‘蓓蕾’蓝靛果中花色苷组成鉴定及抗氧化能力比较分析

对蓝靛果中花色苷的组成进行鉴定，并对其抗氧化能力进行比较分析。实验以蓝靛果（‘蓓蕾’品种）为原料，采用有机溶剂60%乙醇（0.1%盐酸酸化）溶液，超声辅助提取90 min；利用D101大孔树脂对获得的粗提物进行纯化，之后冷冻干燥制得粉末物质。通过pH示差法和福林-酚法分别测定总花色苷含量和总多酚含量，分别为（353.35±0.79）、（474.01±2.12）mg/g；并用高效液相色谱-质谱联用法对花色苷组成进行鉴定，共发现11 种花色苷，其中矢车菊-3-葡萄糖苷为主要花色苷（90.679%）。此外，实验还通过总抗氧化能力测定和2,2’-联氨-二（3-乙基苯并噻唑-6-磺酸）二铵盐自由基、1,1-二苯基-2-三硝基苯肼自由基清除能力测定，比较分析蓝靛果花色苷提取物、矢车菊-3-葡萄糖苷、VC的抗氧化能力，结果表明，3 种物质的抗氧化能力排序为：矢车菊-3-葡萄糖苷＞花色苷提取物＞VC。

Identification and Comparative Analysis of Antioxidant Ability of Anthocyanins in Lonicera caerulea Berry Extracts

The identification of anthocyanins composition and antioxidant activity of Lonicera caerulea berry extracts was investigated in this study. ‘Beilei’ L. caerulea berries were ultrasonically extracted with 60% ethanol containing 0.1% HCl for 90 min. D101 macroporous resin was used to purify the obtained extracts. After that, freeze drying was conducted to obtain powder. The contents of total anthocyanins and polyphenols were determined using pH differential and Folinreagent method as (353.35 ± 0.79) mg/g and (474.01 ± 2.12) mg/g, respectively. High performance liquid chromatographytandem mass spectrometry (HPLC-MS/MS) was used for the identification of anthocyanin composition. A total of 11 individual anthocyanins were found with cyanidin-3-glucoside (90.679%) being the major anthocyanin. Additionally, total antioxidant capacity (T-AOC) and 2,2’-azinobis(3-ethylbenzothiazoline-6-sulphonate) radical (ABTS＋·), 1,1- diphenyl- 2-picrylhydrazyl (DPPH) radical scavenging activities of L. caerulea berry extracts, cyanidin-3-glucoside and VC were comparatively analyzed, and the results revealed a decreasing order of cyanidin-3-glucoside > L. caerulea berry extract > VC.