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Adaptive correction of depth-induced aberrations in multiphoton scanning microscopy using a deformable mirror |
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| Authors: | L Sherman J Y Ye O Albert† & T B Norris |
| Affiliation: | Center for Ultrafast Optical Science, 1006 IST Bldg, University of Michigan, 2200 Bonisteel Blvd, Ann Arbor, MI 48109, U.S.A.;Laboratoire d'Optique Appliquee –ENSTA, Chemin de la Huniere, 91761 Palaiseau Cedex, France |
| Abstract: | We demonstrate adaptive aberration correction for depth‐induced spherical aberration in a multiphoton scanning microscope with a micromachined deformable mirror. Correction was made using a genetic learning algorithm with two‐photon fluorescence intensity feedback to determine the desired shape for an adaptive mirror. For a 40×/0.6 NA long working distance objective, the axial scanning range was increased from 150 mm to 600 mm. |
| Keywords: | Adaptive aberration correction confocal microscopy depth-induced aberration spherical aberration |