Nonlinear and asymmetric open channel characteristics of an ion-selective porin in planar membranes |
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Authors: | A Mathes H Engelhardt |
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Affiliation: | Max-Planck-Institut für Biochemie, Abteilung Molekulare Strukturbiologie, Martinsried, Germany. |
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Abstract: | The open channel characteristics of the bacterial porin Omp32 from Comamonas acidovorans were investigated by means of conductance measurements in planar lipid bilayers of the Montal-Mueller type. Particularly at low salt conditions (< or = 30 mM KCl) Omp32 exhibited some unusual asymmetric and nonlinear functional properties. Current-voltage relationship measurements showed that conductance depends on the orientation of porin molecules and is a nonlinear function of the applied membrane potential. Conductance also depends on the salt concentration in a manner not common to porins and the salt concentration modulates the nonlinearity of conductance-voltage relationships. Omp32 is strongly anion-selective. The nonlinear and asymmetric conductance of the open channel is a new observation in porins. |
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