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绿豆微孔淀粉酶法制备工艺优化研究
引用本文:田龙,王云.绿豆微孔淀粉酶法制备工艺优化研究[J].粮食与油脂,2007(2):23-25.
作者姓名:田龙  王云
作者单位:南阳师范学院生命科学与技术学院,河南南阳,473061
摘    要:以水解率为指标,研究α-淀粉酶与糖化酶复合水解绿豆淀粉制备微孔淀粉工艺条件,通过单因素和正交试验确定酶解最佳工艺条件:α-淀粉酶:糖化酶=1:3,酶用量2.0%,时间20 h,温度42℃,pH4.2。经吸水、吸油率测试,对酶解前后绿豆淀粉进行性质分析表明,微孔淀粉吸水、吸油能力明显大于原淀粉。

关 键 词:微孔淀粉  复合生淀粉酶  绿豆淀粉
文章编号:1008-9578(2007)02-0023-03
修稿时间:2007年1月8日

Study on process optimizing of biological preparation and properties of microporous cassava starch
TIAN Long,WANG Yun.Study on process optimizing of biological preparation and properties of microporous cassava starch[J].Cereals & Oils,2007(2):23-25.
Authors:TIAN Long  WANG Yun
Abstract:In this paper,porous starch is made of cassava starch by enzymatic hydrolysis of complexα-amylase and glucoamylase through mensurating hydrolysis rate.By one factor and orthogonal test the optimum conditions are obtained as follows:α-amylase:glucoamylase=3:1,enzyme amount 2,0%, time 20 hours,temperature 42℃,pH value 4.2.The remaining starch granules after enzymatic hydroly- sis were analyzed by the absorptivity rates of absorbing water and oil show porous starch granule the ab- sorptivity are obviously higher compared with native starch.
Keywords:porous starch  complex amylolytic enzyme  cassava starch
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