化学修饰GC-MS分析海马脂肪酸

以2-氨基-2-甲基丙醇(AMP)为化学修饰试剂,将不饱和脂肪酸羧基修改为含氮杂环,从而避免了链烯基中碳碳双键在EI源中的移动。以气相色谱-EI质谱分析海马脂肪酸,通过解析不饱和脂肪酸AMP化学修饰产物的EI质谱图,确定了海马脂肪酸中碳碳双键的位置,鉴定出海马中13种脂肪酸,不饱和脂肪酸相对含量为75.15%,多不饱和脂肪酸相对含量为26.17%,多不饱和脂肪酸主要为9,12-十八碳二烯酸,9,12,15-十八碳三烯酸和4,7,10,13,16,19-二十二碳六烯酸。

Analysis of Chemically Modification Fatty Acids in Hippocampus by GC-MS

AMP(2-amino-2-methylpropanol)was developed as chemical modifying reagent of fatty acids so as to modify carboxyl group into nitrogen-containing heterocycle which effectively suppressed the migration of double bonds of the alkenyl under the electron impact source.13 fatty acids were identified according to the analysis of GC-EIMS and the position of double bonds of the fatty acids in hippocampus was located by analyzing electron impact spectra of the chemical products modified by AMP. The contents of the unsaturated fatty acids and the polyunsaturated fatty acids are 75.15% and 26.17%, respectively, and the main components of the polyunsaturated fatty acids are 9,12-linoleic acid,9,12,15-linolenic acid and 4,7,10,13,16,19-docosahexenoica cidr espectively.