赤芍拮抗内毒素活性的实验研究

目的:提取分离中药赤芍中拮抗内毒素(LPS)的有效成分并进行药理学活性检测。方法:通过生物传感器, 结合常规的中药分离技术, 分离提取出赤芍拮抗LPS 的有效成分, 应用鲎实验和ELISA 法检测该有效成分对LPS 的中和作用。结果:赤芍中的有效成分与LPS 具有较高的结合作用, 该结合作用具有较强的中和LPS 活性, 在体外能够显著抑制由LPS 介导小鼠RAW 264.7 细胞释放TNF-α。结论:以LPS 为靶点, 应用生物传感器技术, 可快速、有效地提取分离赤芍中具有较强的中和LPS 活性的有效成分。

Experimental study on anti-endotoxin activity of Radix Paeoniae Rubra

AIM: To isolate and extract anti-endotox effective components in Radix Paeoniae Rubra.METHODS: By the biosensor technology, the anti-endotoxin effective components were isolated and extracted with general separate technology of Chinese traditional medicine. Through quantificational mensuration for LPS and inhibition of TNF-αrelease from endotoxin-stimulated cells in vitro, anti-endotoxin activity of Radix Paeoniae Rubra were elucidated.RESULTS: The effective components had high binding capability with LPS.After incubation with endotoxin and cells, the capability showed anti-endotoxin and inhibition of TNF-αrelease from endotoxinstimulated cells in vitro.CONCLUSION: The anti-endotoxin effective components isolated and extracted using LPS as target by the biosensor technology are available in searching anti-endotoxin agents from Radix Paeoniae Rubra.It shows that Radix Paeoniae Rubra has high ability on neutralization with LPS.