3,4,5,6-四羟基口山酮对高糖所致血管内皮依赖性舒张功能减退的保护作用

目的:观察3, 4, 5, 6-四羟基_口山酮对高糖所致血管内皮依赖性舒张功能减退的保护作用。方法:在大鼠离体胸主动脉环, 用高浓度葡萄糖(25mmol°L^-1) 孵育24 h, 检测乙酰胆碱诱导的血管内皮依赖性舒张反应;人脐静脉内皮细胞株(ECV304)用高浓度葡萄糖(30 mmol°L^-1) 培养48 h, 测定培养液中乳酸脱氢酶(LDH) 活性、一氧化氮(NO) 及细胞脂质过氧化物丙二醛(MDA) 含量。结果:3, 4, 5, 6-四羟基_口山酮(1、3 和10 μmol°L^-1) 能显著抑制高糖所致的舒血管效应减退, 并且呈剂量依赖性。3, 4, 5,6-四羟基_口山酮(1、3 和10 μmol°L^-1) 能显著抑制高糖所致的内皮细胞LDH 泄漏、NO 释放和MDA 生成的增加。结论:3, 4, 5, 6-四羟基_口山酮对高糖所致的血管内皮依赖性舒张功能减退有保护作用, 其保护作用与抑制脂质过氧化减少血管内皮细胞损伤有关。

Protective effects of 3,4,5,6-tetrahydroxanthone on damages of endothelium-dependent relaxation induced by high glucose

AIM: To investigate the protective effects of 3, 4, 5, 6-tetrahydroxanthone on damages of endothelium- dependent relaxation induced by high glucose. METHODS: The aortic rings obtained from the rats were exposed to high glucose (25 mmol°L^-1) for 24 h or endothelial cells were incubated with high glucose (30 mmol°L^-1) for 48 h, respectively.Endothelium-dependent relaxation was measured.Activity of lactate dehydrogenase (LDH), contents of malondialdehyde (MDA) and nitric oxide (NO) in the medium were measured.RESULTS: Exposure of aorta rings to high glucose (25 mmol°L^-1) for 24 h caused a marked decrease in endotheliun- dependent relaxation.Pretreatment with 3, 4, 5, 6- tetrahydroxyxanthone (1, 3 or 10 μmol°L^-1) or probucol (10 μmol°L^-1) significantly improved endothelium-dependent relaxation in a dose-dependent manner.The release of LDH, the contents of MDA and NO in the medium marked increased after exposure of HUVEC to high glucose (30mmol°L^-1) for 48 h.Pretreatment with 3, 4, 5, 6-tetrahydroxyxanthone (1, 3 or 10 μmol°L^-1) or probucol (10 μmol°L^-1) significantly decreased the increased release of LDH, the increased content of NO and MDA in the medium.CONCLUSION: 3, 4, 5, 6-tetrahydroxanthone may preserve endothelial dysfunction induced by high glucose, and the protective effects of 3, 4, 5, 6-tetrahydroxanthone may be related to reduction of endothelial cells damages by inhibiting lipid peroxidation.