5-氟脲嘧啶对口腔癌Tb细胞增殖活性及钾通道功能的影响

目的: 观察5-氟脲嘧啶(5-FU)对口腔癌Tb细胞增殖活性以及钾离子通道功能的影响,进一步揭示5-FU的抗肿瘤机理,并初步探讨钾离子通道与肿瘤细胞增殖的关系。方法: 以5-FU作用Tb细胞24h后,应用MTT比色法与平板克隆形成实验观察Tb细胞的增殖活性,同时以全细胞膜片钳技术检测Tb细胞膜电位与跨膜钾电流的改变。结果: 与空白对照组相比,5-FU能明显抑制Tb细胞的增殖活性,并且可使细胞膜电位发生去极化改变,跨膜钾电流显著降低。结论: 5-FU在抑制Tb细胞增殖的同时钾通道功能也显著降低,提示钾离子通道可能参与调控5-FU抑制Tb细胞增殖的过程。

Effects of 5-Fluorouracil on cell proliferation and potassium channel function in oral cancer cell line Tb

AIM: To evaluate the effects of 5-Fluorouracil (5-FU) on cell proliferation and potassium channel function in oral cancer cell line Tb.METHODS: After treatment with 5-FU for 24 h, the proliferation of Tb cells was determined by MTT and clone formation assay, the membrane potential and potassium channel current of Tb cells were recorded by whole-cell patch clamp technique.RESULTS: Compared with the control group (CON), 5-FU inhibited proliferation of the Tb cells.At the same time the membrane was depolarized and the potassium channel current significantly decreased compared with the CON group.CONCLUSION: Potassium channel may play an important role in 5-FU-inhibited the proliferation of Tb cells.