人参须对肝纤维化致小鼠骨丢失的防治作用

目的: 研究人参须对肝纤维化小鼠骨丢失的防治作用。方法: 40 %四氯化碳花生油皮下注射5周致小鼠肝纤维化, 人参须用药6 周后观察与肝损伤相关的各项生化指标和肝脏病理切片结果并测定小鼠右股骨的骨Ca²⁺量和其他骨微量元素以及骨羟脯胺酸的含量。结果: 单用四氯化碳小鼠呈现典型的慢性肝损伤后肝纤维化的改变, 骨重量和骨钙总量及骨羟脯胺酸的含量明显减少;而人参须有明显的护肝及对抗骨丢失作用, 其作用与秋水仙碱相当。结论: 人参须对小鼠肝纤维化及骨丢失有一定预防作用。

Preventive effects of ginseng fiber on hepatic fibrosis induced by bone loss in mice

AIM: To study the relationship between hepatic fibrosis and osteoporosis, and observe the preventive effects of ginseng fiber on bone loss. METHODS: Forty PCR micewere randomly divided into 4 groups. The mice in control group were treated by daily oral gavage with vehicle. The mice in other three groups were given SC injection of 40 %CCl₄ 10 ml·kg^-1 and treated by either daily oral gavage with vehicle, colchicine, or ginsen hair for 42 d. The liver injury indexes were measured and the mineral elements and hydroxyproline of femur were determined. RESULTS: Compared with the control group, the serum enzyme activity of alanine aminotransferase (ALT), aspartic acid aminotransferase (AST) markedly increased and serum albumin (Alb)and A /G distinctly decreased in CCl₄ group whose liver slides also showed typical liver cirrhosis. The dried weight of femur markedly reduced and the bone calcium content and bone hydroxyproline content significantly decreased in CCl₄ group. Bone copper and bone magnesium increased in CCl₄ group. Ginseng fiber markedly decreased the serum enzyme activity of ALT and increased the bone calcium content and bone hydroxyproline content. The preventive effects of ginseng fiber was similar to that of colchicine. CONCLUSION: The bone mass is lost in mice with chronic hepatic injury induced by CCl₄. Ginseng fiber can prevent bone loss and hepatic fibrosis induced by CCl₄ in mice.