不同月龄正常大鼠骨形态计量学参数动态变化的研究

目的:探讨不同月龄正常大鼠骨形态计量学参数变化, 为抗骨质疏松药物研究提供对照依据。方法:4.5 月龄SD 雌性大鼠, 按体重随机分组, 在实验的d 0(4.5 月龄)、d 30 (5.5 月龄)、d 75 (7 月龄)、d 140 (10 月龄) 杀死大鼠取材, 采用体内双荧光标记法, 胫骨上段硬组织包埋切片及松质骨形态计量学分析, 观察不同月龄段大鼠的骨变化情况。结果:正常大鼠4.5～10 月龄, 骨量有一缓慢上升后下降的过程, 但变化辐度不大(P>0.05), 骨量相对稳定, 骨形成和骨吸收有先上升后下降的变化过程(P<0.05) 。结论:4.5～10 月龄的SD 雌性大鼠体重和骨计量学参数变化正常, 选择该月龄段大鼠做模型, 研究药物对骨质疏松的防治是可行的。

Timed changes of bone histomorphometry parameters in normal rats of different months

AIM: To investigate timed changes of bone histomorphometry parameters in normal rats of different months and to provide a control prove for research of drugs of antiosteoporosis.METHODS: Thirty-two female rats at aged 4.5 months were divided by their weight (256.3±25.3 g).Tetracycline derivates and calcein were subcutaneous injected to each rat on two separate occasions where labeled the sites of bone formation.All rats were sacrificed at 0, 30, 75 and 140 days.The undecalcified longitudinal proximal tibial metaphyseal sections were cut and stained for quantitative bone histomorphometriy.RESULTS: The cancellous bone mass increased slowly and then decreased, but there were no significant differences between 4.5 and 10 months.Bone mass kept relatively stable.Both bone for mation and bone resorption increased first and then decreased, but there was a significant difference between 4.5 and 10 months, and bone turnover kept stable.CONCLUSION: The body weight and the bone mass parameters keep relatively stable in SD rats during the 4.5 to 10 months, and these rats can be selected as the models for the study of the drugs of antiosteoporosis.