中国拟青霉对小鼠免疫性肝损伤的保护作用

目的:研究中国拟青霉(CN-802) 对小鼠免疫性肝损伤的保护作用及其对T 淋巴细胞亚群的影响。方法:小鼠尾静脉注射卡介苗加脂多糖(BCG+LPS) 建立免疫性肝损伤模型, 检测其肝、脾重量和转氨酶活性, 并同时测定血清与肝组织中脂质过氧化物(LPO) 含量及T 淋巴细胞亚群数量。结果:CN-802 连用10 d 后可减轻其增大的肝、脾重量指数, 降低小鼠血清中谷丙转氨酶(ALT) 水平, 降低其血清与肝组织中LPO 含量, 并增加外周血中T 细胞亚群CD₄ 数量和CD₄/CD₈ 比值。结论:CN-802 对小鼠免疫性肝损伤模型有保护作用, 其作用可能与调节细胞免疫有关。

Protective effects of CN802 on immunological liver injury in mice

AIM: To study the protect effects of paecilomyceps sinensis sp nov (CN-802) on immunological liver injury in mice which induced by BCG+LPS. METHODS: LPS was injected into mice via the tail vein pretreated with BCG.The activity of serum ALT, the weighing indexes of liver and spleen, the levels of LPO in serum and hepatic tissues, and the numbers of subgroup of T lymphocyte were determined respectively.RESULTS: CN-802 at dose of 1.0 g°kg^-1 °d^-1 decreased the serum ALT level, the liver and spleen-enlarged index and the LPO levels in serum and hepatic tissues.Analysis of CD₄/CD₈ in the peripheral blood showed that CN-802 increased the number of CD₄ lymphocyte and the ratio of CD₄/CD₈.CONCLUTION: Pretreatment with CN-802 prevents the immunological liver injury induced by BCG+LPG, and the effect of CN802 may be related with the regulatory of immune cells.