三种荧光分光光度法测定肿瘤组织中阿霉素含量的研究

目的: 通过比较测定肿瘤组织中阿霉素(ADR)含量的3 种荧光分光光度法, 建立简便、快捷的测定肿瘤组织中阿霉素含量的荧光分光光法。方法: 分别采用正丁醇法、硝酸银法、酸性异丙醇法测定水溶液及肿瘤组织匀浆中ADR 的含量, 比较荧光光谱曲线、回收率和灵敏度。结果: 硝酸银法测定瘤组织匀浆中的ADR 含量, 背景荧光值低, 线性范围0.2～0.8 mg·L^-1, 最低检出量0.15 mg·L^-1, 回收率超过97 %以上。酸性异丙醇法与正丁醇法测定瘤组织中的ADR 含量, 背景荧光值高, 回收率分别<40 %和50 %。结论: 硝酸银法适合肿瘤组织中的ADR 的含量测定, 该方法准确、灵敏、可靠。

Research on three fluorospectrophotometry methods for determination of concentration of adriamycin in tumor tissue

AIM: To establish a simple and rapid fluorospectrophotometry method for the determination of concentration of adriamycin (ADR)in tumor tissue by comparing with three fluorospectrophotometry methods. METHODS: The concentrations of ADR in water and in tumor tissue were measured by three fluorospectrophotometry methods: n-butanol, silver nitrate and acidityisopropanol assay.The fluorescence spectrum curve, recovery and sensitivity were compared.RESULTS: The method of silver nitrate for determination of concentration of ADR in tumor tissue had low background tissue fluorescence.The linear range was 0.2-0.8 mg·L^-1, the limit of detection was 0 .15 mg·L^-1, and the recovery was over 97% by silver nitrate method .The method of acidity- isopropanol and n-butanol for determination of ADR in tumor-tissue fluorescence and low recovery which was <40 % by acidity-isopropanol assay and <50 % by n-butanol assay, respectively.CONCLUSION: The method of silver nitrate shows accurate, sensitive and reliable characterization for determination of concentration of ADR in tumor tissue.