大鼠肺泡 、腹腔巨噬细胞甘露糖受体与配体的亲和力比较

目的: 探讨来源于不同组织肺泡、腹腔巨噬细胞甘露糖受体(macrophagemannosereceptor,MMR)与配体的亲和力差异。方法: 采用支气管肺泡灌洗和腹腔冲洗法获得肺泡和腹腔巨噬细胞(macrophage,Mφ),经纯化、鉴定后与异硫氰酸荧光标记的甘露糖基化牛血清白蛋白(M-FITC-BSA)孵育20min,分别加入D-甘露糖、D-半乳糖、EDTA,采用荧光显微镜和流式细胞仪检测甘露糖受体(mannosereceptor,MR)与配体的结合情况。结果: Mφ上存在MR,MR与配体的结合属于Ca²⁺依赖性,其结合可被D-甘露糖、EDTA抑制,而不受D-半乳糖的抑制;腹腔MMR与配体的亲和力高于肺泡MMR。结论: 腹腔MMR与配体的亲和力高于肺泡MMR。

Comparison of affinity of mannose receptorof peritoneal macrophage and alveolarmacrophage and its ligands in rats

AIM: To evaluate the affinity of the man-nose receptorof peritoneal macrophage and pulmonary macrophage and theirligands.METHODS: Two kinds of macrophage were acquired through Irrigation of peritoneal cavity and bronchoalveolarlavage(BAL).Afterpurifica-tion and identification,macrophage were incubated with fluorescent isothiocyanate(FITC)-labeled mannosylated albumin(M-FITC-BSA,Sigma A7790)for20 minites,the affinity of Mrand its ligands are measured by flow cytometry(FCM)and fluorescene microscope.RESULTS: There existed Mrin macrophage.The binding of Mrand its ligandswas calcium dependent,which was inhibited by the existence of D-mannose and EDTA,but not by D-galactose.The affinity of peritoneal macrophage Mrand its ligands were greaterthan that of the pulmo-nary macrophage Mrand its ligands.CONCLUSION: The affinity of peritoneal macrophage Mrand its ligands are greaterthan that of the pulmonary macrophage Mrand its ligands.