| 阿霉素诱导小鼠MCF-7 乳腺癌细胞凋亡和抑制增殖的体内实验研究 |
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| 引用本文： | 王文武,欧阳学农,姜浩. 阿霉素诱导小鼠MCF-7 乳腺癌细胞凋亡和抑制增殖的体内实验研究[J]. 金属学报, 2005, 10(6): 674-676 |
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| 作者姓名： | 王文武 欧阳学农 姜浩 |
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| 作者单位： | 国家中西医结合肿瘤重点专科, 南京军区福州总医院肿瘤科, 福州350025, 福建;1.南华大学附属第一医院肿瘤科, 衡阳421001, 湖南 |
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| 基金项目： | 湖南省卫生厅课题基金项目(NO2001-Y58) |
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| 摘 要： | 目的: 探讨阿霉素对荷瘤鼠MCF-7 乳腺癌细胞凋亡和增殖的影响。方法: 建立小鼠MCF-7 乳腺癌细胞模型, 用TUNEL 法和免疫组化法检测阿霉素作用下MCF-7 乳腺癌细胞的凋亡和PCNA 表达的变化。结果: 1.25 mg·kg-1 阿霉素的抑瘤率为43 %。MCF-7 乳腺癌细胞的凋亡指数为1.93 %, 较荷瘤对照组的1.11 %明显升高(P <0.05)。增殖指数为35.75 %, 较荷瘤对照组的56.38 %明显下降(P <0.01)。结论: 阿霉素有诱导小鼠MCF-7 乳腺癌细胞凋亡和抑制其增殖的作用。
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| 关 键 词： | 阿霉素 乳腺肿瘤 细胞凋亡 增殖细胞核抗原 |
| 收稿时间： | 2005-02-28 |
| 修稿时间： | 2005-04-06 |
In vivo study on apoptosis and cell proliferation induced by adriamycin in mice with MCF-7 breast cancer |
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| WANG Wen-wu,OU-YANG Xue-nong,JIANG Hao. In vivo study on apoptosis and cell proliferation induced by adriamycin in mice with MCF-7 breast cancer[J]. Acta Metallurgica Sinica, 2005, 10(6): 674-676 |
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| Authors: | WANG Wen-wu OU-YANG Xue-nong JIANG Hao |
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| Affiliation: | Department of Oncology, NanJing Military Command Fuzhou General Hospital, Fuzhou 350025, Fujian, China;1.Department of Oncology, the First Hospital Affiliated to Nanhua Uniuersity, Hengyang 421000, Hunan, China |
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| Abstract: | AIM: To observe the apoptosis and cell proliferation induced by adriamycin in mice with breast cancer. METHODS: Therapeutic effect of 1.25 mg·kg-1 adriamycin (ADR)on mice MCF-7 breast cancer was observed in vivo. Apoptosis and PCNA of tumor cells were also studied by TUNEL and immune his to chemical methods. RESULTS: In the control group, the apoptotic cell index (AI)in tumor cell was 1.11 %and PCNA index (PI)was 56.38 %.In the ADR group, the rate of tumor inhibition was 43 % and the AI was 1.93 % (P <0.05).The 35.75 % of PI in ADR group was lower than that in the control group (P <0.01). CONCLUSION: Adriamycin may induce apoptosis and reduce cell proliferation. |
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| Keywords: | adriamycin breast neoplasm apoptosis PCNA |
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