超微粉碎辅助酶解法制备海参冻干粉的生物活性分析

以超微粉碎辅助酶解法制备的海参（Cucumaria frondosa）冻干粉为研究对象，通过DPPH自由基清除率、羟基自由基清除率和还原力、HeLa细胞增殖抑制率、小鼠转棒实验及血乳酸（lactic acid,LA）含量和环磷酰胺（cyclophosphamide,CY）诱导的免疫缺陷小鼠实验及免疫球蛋白G(immunoglobulin G,IgG)含量，分别研究海参冻干粉的抗氧化能力、抗肿瘤、抗疲劳和提高免疫力的能力。结果表明，DPPH自由基清除率的IC50为12.46 mg/mL，羟基自由基清除率的IC50为15.22 mg/mL，还原力为0.35，表明海参冻干粉具有抗氧化能力。海参冻干粉对HeLa细胞增殖抑制率的IC50为556.2μg/mL，表现出抗肿瘤能力。通过灌胃海参冻干粉，小鼠转棒运动时间随着剂量升高而延长，运动后高剂量组的LA含量（19.15 mmol/L）较低，说明小鼠运动耐力提高，且CY诱导的免疫缺陷小鼠降低了体质量的下降速率，高剂量组IgG含量恢复到11.98 mg/mL。综上，海参冻干粉具有抗氧化、抗肿瘤抗疲劳和提高免疫力的多功能活性潜力。

Bioactivity of Sea Cucumber Freeze-Dried Powder Prepared by Vibration-Assisted Milling Combined with Enzymatic Hydrolysis

Sea cucumber freeze-dried powder was prepared by vibration-assisted milling combined with enzymatic hydrolysis.The antioxidant activity of the sea cucumber freeze-dried powder was evaluated based on the DPPH and hydroxyl radical scavenging rates and reducing power.The anti-tumor activity was examined based on the proliferation inhibition rate of HeLa cells.The rotarod test of mice and the blood lactic acid（LA）content were employed to measure the anti-fatigue activity.The mouse model of cyclophosphamide（CY）-induced immune deficiency and the immunoglobulin G（IgG）content were employed to assess the immunity-enhancing activity of the powder.The prepared sea cucumber freeze-dried powder showed the IC50 of 12.46 mg/mL and 15.22 mg/mL for scavenging DPPH and hydroxyl free radicals，respectively，and the reducing power of 0.35，which indicated that the sea cucumber freeze-dried powder possessed antioxidant activity.The sea cucumber freeze-dried powder showed the IC50 of 556.2 μg/mL on the proliferation of HeLa cells，demonstrating anti-tumor activity.The administration of sea cucumber freeze-dried powder prolonged the duration of rotarod exercise of mice in a dose-dependent manner，and the LA content（19.15 mmol/L）in the high-dose group piled up less after exercise，which suggested that the powder improved the exercise endurance of mice.Furthermore，the sea cucumber freeze-dried powder slowed down the body weight loss in the mouse model of CY-induced immune deficiency in a dose-dependent manner，and the IgG content in the high-dose group was restored to 11.98 mg/mL.In conclusion，sea cucumber freeze-dried powder has antioxidant，anti-tumor，anti-fatigue，and immunity-enhancing activities.