丹参对博莱霉素所致大鼠肺纤维化保护作用的实验研究

目的: 探讨丹参对博莱霉素所致大鼠肺纤维化的保护作用及其作用机理。方法: 通过气管内注射博莱霉素复制大鼠肺纤维化模型, 在造模后的d 1 和 d 7 开始给予丹参注射液治疗, 分别在 d 7、d 28 处死大鼠, 对肺组织匀浆中的脂质过氧化物(MDA)、谷胱甘肽(GSH)、羟脯氨酸(HYP) 和肺组织病理学变化测量。结果: 在造模后 d 1 给予丹参注射液治疗能抑制实验性肺纤维化大鼠的肺组织匀浆中的MDA、HYP 异常升高和GSH 的下降, 减轻其肺部的病理损害;在造模后 d 7 给予丹参注射液治疗,丹参治疗组与模型组上述观察指标比较无统计学差异。结论: 丹参注射液可预防大鼠肺纤维化, 对已形成的肺纤维化无逆转作用。

Study of Salvia miltiorrhiza on bleomycin-induced pulmonary fibrosis in mice

AIM: To study protective effects of Salvia miltiorrhiza on bleomycin (BLM)-induced pulmo-nary fibrosis in mice. METHODS: Animal model with pulmonary fibrosis in SD mice was replicated by intratra-cheal injections of bleomycin and Salvia miltiorrhiza in-jection were given after replicated 7th day. Ratswere exe-cuted in 7th and 28th day, MDA, GSH andHYP in lung tissue bomogenate and the lung histopathological change were detected to investigate the effects of Salvia miltior-rhiza injection on lung fibrisis form in different stages. RESULTS: Salviae miltiorrhizae injection given after replicated first day restrained the increase of MDA and HYP, and decreased the GSH of lung tissue bomogenate, and lighten pathological lesion.Salvia miltiorrhiza injec-tion given after replicated the 7th day had no statistical difference between Salvia miltiorrhiza therapy groups and model group.CONCLUSION: Salvia miltiorrhiza injec-tion can prevent mice pulmonary fibrosis from and has no side effects on formative rat pulmonary fibrosis.